Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 29077..29602 Replicon plasmid pO157
Accession NZ_CP018246
Organism Escherichia coli strain 472

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag BFL23_RS30385 Protein ID WP_001159868.1
Coordinates 29077..29382 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag BFL23_RS30390 Protein ID WP_000813634.1
Coordinates 29384..29602 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL23_RS30355 24316..25056 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
BFL23_RS30360 25341..26273 - 933 Protein_22 RepB family plasmid replication initiator protein -
BFL23_RS30375 26334..27547 + 1214 WP_187655777.1 IS3 family transposase -
BFL23_RS30380 28270..29076 - 807 WP_000016989.1 site-specific integrase -
BFL23_RS30385 29077..29382 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
BFL23_RS30390 29384..29602 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BFL23_RS30400 30061..30744 + 684 WP_010891291.1 DNA-binding protein -
BFL23_RS30405 30741..31361 + 621 WP_001248529.1 hypothetical protein -
BFL23_RS30410 31358..31558 + 201 WP_000708307.1 hypothetical protein -
BFL23_RS30445 33320..33589 + 270 WP_001241949.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP / espP 1..95341 95341
- inside IScluster/Tn - - 26334..33263 6929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T69167 WP_001159868.1 NZ_CP018246:c29382-29077 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T69167 NZ_CP018246:c29382-29077 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT69167 WP_000813634.1 NZ_CP018246:c29602-29384 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT69167 NZ_CP018246:c29602-29384 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References