Detailed information of TA system TA069166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5319065..5319660
• Replicon: chromosome
• Accession: NZ_CP018245
• Organism: Escherichia coli strain 472

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: BFL23_RS29315
• Protein ID: WP_000239579.1
• Coordinates: 5319310..5319660 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: BFL23_RS29310
• Protein ID: WP_001223210.1
• Coordinates: 5319065..5319316 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL23_RS29300	5314730..5318509	+	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-
BFL23_RS29305	5318512..5318853	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
BFL23_RS29310	5319065..5319316	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
BFL23_RS29315	5319310..5319660	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
BFL23_RS29320	5319740..5320270	-	531	WP_000055075.1	inorganic diphosphatase	-
BFL23_RS29325	5320580..5321536	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
BFL23_RS29330	5321676..5323178	+	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
BFL23_RS29335	5323192..5324214	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T69166 WP_000239579.1 NZ_CP018245:5319310-5319660 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T69166 NZ_CP018245:5319310-5319660 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT69166 WP_001223210.1 NZ_CP018245:5319065-5319316 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT69166 NZ_CP018245:5319065-5319316 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8