Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4402448..4403079 Replicon chromosome
Accession NZ_CP018245
Organism Escherichia coli strain 472

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag BFL23_RS24565 Protein ID WP_001259385.1
Coordinates 4402804..4403079 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BFL23_RS24560 Protein ID WP_000593555.1
Coordinates 4402448..4402807 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL23_RS24530 4397561..4398034 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
BFL23_RS24535 4398031..4398312 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
BFL23_RS24540 4398389..4399747 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
BFL23_RS24545 4399740..4401248 + 1509 WP_001301773.1 carbohydrate kinase -
BFL23_RS24550 4401238..4401507 + 270 WP_000933060.1 HPr family phosphocarrier protein -
BFL23_RS24555 4401539..4402398 + 860 Protein_4346 class II aldolase -
BFL23_RS24560 4402448..4402807 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BFL23_RS24565 4402804..4403079 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
BFL23_RS24570 4403152..4404276 - 1125 WP_001301659.1 ABC transporter permease -
BFL23_RS24575 4404276..4407011 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
BFL23_RS24580 4407008..4408075 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T69162 WP_001259385.1 NZ_CP018245:c4403079-4402804 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T69162 NZ_CP018245:c4403079-4402804 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT69162 WP_000593555.1 NZ_CP018245:c4402807-4402448 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT69162 NZ_CP018245:c4402807-4402448 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References