Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4359849..4360649 Replicon chromosome
Accession NZ_CP018245
Organism Escherichia coli strain 472

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag BFL23_RS24315 Protein ID WP_000342448.1
Coordinates 4359849..4360376 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag BFL23_RS24320 Protein ID WP_001277108.1
Coordinates 4360383..4360649 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL23_RS24285 4354924..4355691 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
BFL23_RS24290 4355688..4356965 - 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
BFL23_RS24295 4356962..4357888 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
BFL23_RS24300 4357936..4359045 - 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
BFL23_RS24310 4359469..4359852 + 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
BFL23_RS24315 4359849..4360376 - 528 WP_000342448.1 GNAT family N-acetyltransferase Toxin
BFL23_RS24320 4360383..4360649 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
BFL23_RS24325 4360799..4361902 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
BFL23_RS24330 4362173..4363027 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
BFL23_RS24335 4363272..4364330 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
BFL23_RS24340 4364323..4364991 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T69161 WP_000342448.1 NZ_CP018245:c4360376-4359849 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T69161 NZ_CP018245:c4360376-4359849 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT69161 WP_001277108.1 NZ_CP018245:c4360649-4360383 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT69161 NZ_CP018245:c4360649-4360383 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References