Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2195490..2195961 Replicon chromosome
Accession NZ_CP018245
Organism Escherichia coli strain 472

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag BFL23_RS12275 Protein ID WP_001303511.1
Coordinates 2195683..2195961 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag BFL23_RS12270 Protein ID WP_001302048.1
Coordinates 2195490..2195681 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL23_RS12230 2191231..2191971 - 741 WP_000788938.1 ATP-binding protein -
BFL23_RS12235 2191978..2192940 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
BFL23_RS12240 2192963..2193388 - 426 WP_000693943.1 Rha family transcriptional regulator -
BFL23_RS12245 2193385..2193669 - 285 WP_072617017.1 transcriptional regulator -
BFL23_RS12265 2195098..2195469 + 372 WP_001169686.1 hypothetical protein -
BFL23_RS12270 2195490..2195681 + 192 WP_001302048.1 hypothetical protein Antitoxin
BFL23_RS12275 2195683..2195961 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BFL23_RS12285 2196314..2196613 + 300 WP_001240334.1 hypothetical protein -
BFL23_RS12290 2196685..2196903 + 219 WP_001171930.1 hypothetical protein -
BFL23_RS12300 2197472..2197660 + 189 WP_000449192.1 cell division inhibition protein DicB -
BFL23_RS12305 2197657..2197848 + 192 WP_001090200.1 DUF1482 family protein -
BFL23_RS12310 2197941..2200412 + 2472 WP_000048458.1 exonuclease -
BFL23_RS12315 2200485..2200736 + 252 WP_000005552.1 excisionase family protein -
BFL23_RS31905 2200771..2200958 + 188 Protein_2163 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2189020..2197848 8828


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T69146 WP_001303511.1 NZ_CP018245:2195683-2195961 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T69146 NZ_CP018245:2195683-2195961 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT69146 WP_001302048.1 NZ_CP018245:2195490-2195681 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT69146 NZ_CP018245:2195490-2195681 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References