Detailed information of TA system TA069111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2098515..2098986
• Replicon: chromosome
• Accession: NZ_CP018243
• Organism: Escherichia coli strain 350

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: BFL21_RS11490
• Protein ID: WP_001303511.1
• Coordinates: 2098708..2098986 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: BFL21_RS11485
• Protein ID: WP_001302048.1
• Coordinates: 2098515..2098706 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL21_RS11440	2093722..2094306	-	585	WP_000206793.1	DUF551 domain-containing protein	-
BFL21_RS11445	2094363..2094758	-	396	WP_001118159.1	DUF977 family protein	-
BFL21_RS11450	2094774..2095543	-	770	Protein_2013	DUF1627 domain-containing protein	-
BFL21_RS11460	2095569..2096309	-	741	WP_000788938.1	ATP-binding protein	-
BFL21_RS11465	2096316..2097278	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
BFL21_RS11470	2097301..2097726	-	426	WP_000693943.1	Rha family transcriptional regulator	-
BFL21_RS11475	2097723..2098025	-	303	WP_000172738.1	transcriptional regulator	-
BFL21_RS11480	2098123..2098494	+	372	WP_001169686.1	hypothetical protein	-
BFL21_RS11485	2098515..2098706	+	192	WP_001302048.1	hypothetical protein	Antitoxin
BFL21_RS11490	2098708..2098986	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFL21_RS11500	2099339..2099638	+	300	WP_072143447.1	hypothetical protein	-
BFL21_RS11505	2099710..2099928	+	219	WP_001171930.1	hypothetical protein	-
BFL21_RS11515	2100497..2100685	+	189	WP_000449192.1	cell division inhibition protein DicB	-
BFL21_RS11520	2100682..2100873	+	192	WP_001090200.1	DUF1482 family protein	-
BFL21_RS11525	2100966..2103437	+	2472	WP_000048458.1	exonuclease	-
BFL21_RS11530	2103510..2103761	+	252	WP_000005552.1	excisionase family protein	-
BFL21_RS30960	2103796..2103980	+	185	Protein_2027	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2057287..2113866	56579

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T69111 WP_001303511.1 NZ_CP018243:2098708-2098986 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T69111 NZ_CP018243:2098708-2098986 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT69111 WP_001302048.1 NZ_CP018243:2098515-2098706 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT69111 NZ_CP018243:2098515-2098706 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0