Detailed information of TA system TA069105

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1407079..1407874
• Replicon: chromosome
• Accession: NZ_CP018243
• Organism: Escherichia coli strain 350

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: BFL21_RS07400
• Protein ID: WP_000854906.1
• Coordinates: 1407500..1407874 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0B0VS41
• Locus tag: BFL21_RS07395
• Protein ID: WP_001280954.1
• Coordinates: 1407079..1407453 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL21_RS07350	1402434..1403339	+	906	WP_000203541.1	chemotaxis protein	-
BFL21_RS07355	1403336..1404406	+	1071	WP_000102669.1	patatin-like phospholipase family protein	-
BFL21_RS07370	1404746..1405564	+	819	WP_001234682.1	DUF945 domain-containing protein	-
BFL21_RS07375	1405655..1406140	+	486	WP_000214398.1	antirestriction protein	-
BFL21_RS07380	1406156..1406632	+	477	WP_001186738.1	RadC family protein	-
BFL21_RS07385	1406695..1406916	+	222	WP_001220314.1	DUF987 domain-containing protein	-
BFL21_RS07395	1407079..1407453	+	375	WP_001280954.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BFL21_RS07400	1407500..1407874	+	375	WP_000854906.1	TA system toxin CbtA family protein	Toxin
BFL21_RS07405	1407871..1408362	+	492	WP_000976858.1	hypothetical protein	-
BFL21_RS07410	1408374..1408571	+	198	WP_000839282.1	DUF957 domain-containing protein	-
BFL21_RS07415	1408656..1409498	+	843	WP_001280481.1	DUF4942 domain-containing protein	-
BFL21_RS07425	1409969..1410907	+	939	WP_000351284.1	glyoxylate/hydroxypyruvate reductase GhrA	-
BFL21_RS07430	1410962..1411699	+	738	WP_000283664.1	zinc-binding phosphatase	-
BFL21_RS07435	1411723..1412277	+	555	WP_001001917.1	molecular chaperone YcdY	-
BFL21_RS07440	1412379..1412870	+	492	WP_001301587.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14087.14 Da
• Isoelectric Point: 7.7761

>T69105 WP_000854906.1 NZ_CP018243:1407500-1407874 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T69105 NZ_CP018243:1407500-1407874 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13718.51 Da
• Isoelectric Point: 6.6249

>AT69105 WP_001280954.1 NZ_CP018243:1407079-1407453 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT69105 NZ_CP018243:1407079-1407453 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0VS41