Detailed information of TA system TA069101

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1119380..1120215
• Replicon: chromosome
• Accession: NZ_CP018243
• Organism: Escherichia coli strain 350

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: BFL21_RS05645
• Protein ID: WP_032107687.1
• Coordinates: 1119838..1120215 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BFL21_RS05640
• Protein ID: WP_033805992.1
• Coordinates: 1119380..1119748 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL21_RS31465	1114667..1115124	+	458	Protein_991	hypothetical protein	-
BFL21_RS05600	1115249..1115788	+	540	WP_001104020.1	DUF4339 domain-containing protein	-
BFL21_RS05605	1116058..1116471	+	414	WP_000789535.1	hypothetical protein	-
BFL21_RS30710	1116569..1116792	+	224	Protein_994	DUF905 family protein	-
BFL21_RS05615	1116882..1117700	+	819	WP_033805991.1	DUF945 domain-containing protein	-
BFL21_RS31470	1117700..1117873	+	174	WP_001684385.1	hypothetical protein	-
BFL21_RS05625	1118039..1118518	+	480	WP_001684386.1	antirestriction protein	-
BFL21_RS05630	1118534..1119010	+	477	WP_001186745.1	RadC family protein	-
BFL21_RS05635	1119079..1119300	+	222	WP_001684649.1	DUF987 domain-containing protein	-
BFL21_RS05640	1119380..1119748	+	369	WP_033805992.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BFL21_RS05645	1119838..1120215	+	378	WP_032107687.1	type IV toxin-antitoxin system toxin CbtA	Toxin
BFL21_RS05650	1120212..1120700	+	489	WP_001684187.1	hypothetical protein	-
BFL21_RS05655	1120712..1120888	+	177	WP_001684188.1	DUF957 domain-containing protein	-
BFL21_RS05660	1120994..1121836	+	843	WP_001684189.1	DUF4942 domain-containing protein	-
BFL21_RS05670	1122327..1122545	-	219	WP_001040187.1	translation initiation factor IF-1	-
BFL21_RS05680	1122830..1123534	-	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13903.98 Da
• Isoelectric Point: 7.7785

>T69101 WP_032107687.1 NZ_CP018243:1119838-1120215 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTCSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGVKQ

Nucleotide

• Length: 378 bp

>T69101 NZ_CP018243:1119838-1120215 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCTGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13578.33 Da
• Isoelectric Point: 6.7384

>AT69101 WP_033805992.1 NZ_CP018243:1119380-1119748 [Escherichia coli]
VSDTLHETNHPDGNNNSPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGQFSDADVYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSKTKK

Nucleotide

• Length: 369 bp

>AT69101 NZ_CP018243:1119380-1119748 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATCACCCCGACGGCAATAACAACAGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGTATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCAAAACGAAAAAGTAA