Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 71643..72202 Replicon plasmid pO157
Accession NZ_CP018242
Organism Escherichia coli strain 319

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag BFL24_RS30240 Protein ID WP_000421248.1
Coordinates 71927..72202 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag BFL24_RS30235 Protein ID WP_001178089.1
Coordinates 71643..71927 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL24_RS30180 66834..67394 + 561 WP_072640423.1 fertility inhibition protein FinO -
BFL24_RS30185 67527..67739 + 213 WP_001302189.1 ANR family transcriptional regulator -
BFL24_RS30190 67984..68445 + 462 WP_001233853.1 thermonuclease family protein -
BFL24_RS30195 68491..68700 + 210 WP_001302200.1 hemolysin expression modulator Hha -
BFL24_RS30200 68738..69076 + 339 WP_000766796.1 hypothetical protein -
BFL24_RS30205 69316..69570 + 255 WP_000083831.1 replication regulatory protein RepA -
BFL24_RS30215 69806..69880 + 75 WP_001370046.1 RepA leader peptide Tap -
BFL24_RS30220 69873..70730 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
BFL24_RS30235 71643..71927 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
BFL24_RS30240 71927..72202 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BFL24_RS30245 72297..72503 + 207 WP_001105064.1 hypothetical protein -
BFL24_RS30255 72603..72818 - 216 Protein_77 transposase -
BFL24_RS30260 72862..73428 - 567 Protein_78 IS3-like element IS600 family transposase -
BFL24_RS30265 73428..73844 - 417 Protein_79 IS91 family transposase -
BFL24_RS30270 73844..74029 - 186 WP_001302179.1 hypothetical protein -
BFL24_RS30275 74206..76416 + 2211 WP_000592771.1 catalase/peroxidase KatP -
BFL24_RS30280 76460..76849 + 390 WP_001172748.1 cytochrome b562 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..92495 92495


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T69095 WP_000421248.1 NZ_CP018242:71927-72202 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T69095 NZ_CP018242:71927-72202 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT69095 WP_001178089.1 NZ_CP018242:71643-71927 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT69095 NZ_CP018242:71643-71927 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References