Detailed information of TA system TA069087

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4587723..4588524
• Replicon: chromosome
• Accession: NZ_CP018241
• Organism: Escherichia coli strain 319

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E0XS49
• Locus tag: BFL24_RS25160
• Protein ID: WP_001094437.1
• Coordinates: 4588147..4588524 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0E0XTQ1
• Locus tag: BFL24_RS25155
• Protein ID: WP_001390338.1
• Coordinates: 4587723..4588100 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL24_RS25115	4584838..4584996	+	159	WP_001323397.1	DUF905 family protein	-
BFL24_RS25125	4585151..4585969	+	819	WP_001535681.1	DUF945 domain-containing protein	-
BFL24_RS25135	4586311..4586784	+	474	WP_000855064.1	antirestriction protein	-
BFL24_RS25140	4586800..4587276	+	477	WP_001535682.1	RadC family protein	-
BFL24_RS25145	4587339..4587560	+	222	WP_000692345.1	DUF987 domain-containing protein	-
BFL24_RS25155	4587723..4588100	+	378	WP_001390338.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BFL24_RS25160	4588147..4588524	+	378	WP_001094437.1	TA system toxin CbtA family protein	Toxin
BFL24_RS25165	4588521..4589009	+	489	WP_053920163.1	hypothetical protein	-
BFL24_RS25170	4589029..4589226	+	198	WP_000772032.1	DUF957 domain-containing protein	-
BFL24_RS25175	4589311..4590156	+	846	WP_001274561.1	DUF4942 domain-containing protein	-
BFL24_RS25180	4590440..4590820	+	381	WP_001171554.1	IS66 family insertion sequence hypothetical protein	-
BFL24_RS25185	4590817..4591164	+	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
BFL24_RS25190	4591214..4592752	+	1539	WP_000998051.1	IS66-like element ISEc8 family transposase	-
BFL24_RS31405	4593070..4593377	+	308	Protein_4458	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.90 Da
• Isoelectric Point: 7.3223

>T69087 WP_001094437.1 NZ_CP018241:4588147-4588524 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T69087 NZ_CP018241:4588147-4588524 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13745.44 Da
• Isoelectric Point: 5.0463

>AT69087 WP_001390338.1 NZ_CP018241:4587723-4588100 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYAYLAVYPTPAEPAITV

Nucleotide

• Length: 378 bp

>AT69087 NZ_CP018241:4587723-4588100 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XS49

Antitoxin

• AlphaFold DB: A0A0E0XTQ1