Detailed information of TA system TA069068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2188950..2189421
• Replicon: chromosome
• Accession: NZ_CP018241
• Organism: Escherichia coli strain 319

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: BFL24_RS11995
• Protein ID: WP_001303511.1
• Coordinates: 2189143..2189421 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: BFL24_RS11990
• Protein ID: WP_001302048.1
• Coordinates: 2188950..2189141 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL24_RS11945	2184157..2184741	-	585	WP_000206793.1	DUF551 domain-containing protein	-
BFL24_RS11950	2184798..2185193	-	396	WP_001118159.1	DUF977 family protein	-
BFL24_RS11955	2185209..2185978	-	770	Protein_2104	DUF1627 domain-containing protein	-
BFL24_RS11965	2186004..2186744	-	741	WP_000788938.1	ATP-binding protein	-
BFL24_RS11970	2186751..2187713	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
BFL24_RS11975	2187736..2188161	-	426	WP_000693943.1	Rha family transcriptional regulator	-
BFL24_RS11980	2188158..2188460	-	303	WP_000172738.1	transcriptional regulator	-
BFL24_RS11985	2188558..2188929	+	372	WP_001169686.1	hypothetical protein	-
BFL24_RS11990	2188950..2189141	+	192	WP_001302048.1	hypothetical protein	Antitoxin
BFL24_RS11995	2189143..2189421	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFL24_RS12005	2189774..2190073	+	300	WP_072143447.1	hypothetical protein	-
BFL24_RS12010	2190145..2190363	+	219	WP_001171930.1	hypothetical protein	-
BFL24_RS12020	2190932..2191120	+	189	WP_000449192.1	cell division inhibition protein DicB	-
BFL24_RS12025	2191117..2191308	+	192	WP_001090200.1	DUF1482 family protein	-
BFL24_RS12030	2191401..2193872	+	2472	WP_000048458.1	exonuclease	-
BFL24_RS12035	2193945..2194196	+	252	WP_000005552.1	excisionase family protein	-
BFL24_RS31755	2194231..2194415	+	185	Protein_2118	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2147721..2204301	56580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T69068 WP_001303511.1 NZ_CP018241:2189143-2189421 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T69068 NZ_CP018241:2189143-2189421 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT69068 WP_001302048.1 NZ_CP018241:2188950-2189141 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT69068 NZ_CP018241:2188950-2189141 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0