Detailed information of TA system TA069058

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1109277..1110112
• Replicon: chromosome
• Accession: NZ_CP018241
• Organism: Escherichia coli strain 319

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: BFL24_RS05565
• Protein ID: WP_032107687.1
• Coordinates: 1109735..1110112 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BFL24_RS05560
• Protein ID: WP_033805992.1
• Coordinates: 1109277..1109645 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL24_RS31940	1104560..1105017	+	458	Protein_980	hypothetical protein	-
BFL24_RS05520	1105142..1105685	+	544	Protein_981	DUF4339 domain-containing protein	-
BFL24_RS05525	1105955..1106368	+	414	WP_000789535.1	hypothetical protein	-
BFL24_RS31025	1106466..1106689	+	224	Protein_983	DUF905 family protein	-
BFL24_RS05535	1106779..1107597	+	819	WP_033805991.1	DUF945 domain-containing protein	-
BFL24_RS31945	1107597..1107770	+	174	WP_001684385.1	hypothetical protein	-
BFL24_RS05545	1107936..1108415	+	480	WP_001684386.1	antirestriction protein	-
BFL24_RS05550	1108431..1108907	+	477	WP_001186745.1	RadC family protein	-
BFL24_RS05555	1108976..1109197	+	222	WP_001684649.1	DUF987 domain-containing protein	-
BFL24_RS05560	1109277..1109645	+	369	WP_033805992.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BFL24_RS05565	1109735..1110112	+	378	WP_032107687.1	type IV toxin-antitoxin system toxin CbtA	Toxin
BFL24_RS05570	1110109..1110597	+	489	WP_001684187.1	hypothetical protein	-
BFL24_RS05575	1110609..1110785	+	177	WP_001684188.1	DUF957 domain-containing protein	-
BFL24_RS05580	1110891..1111733	+	843	WP_072640308.1	DUF4942 domain-containing protein	-
BFL24_RS05590	1112224..1112442	-	219	WP_001040187.1	translation initiation factor IF-1	-
BFL24_RS05600	1112727..1113431	-	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13903.98 Da
• Isoelectric Point: 7.7785

>T69058 WP_032107687.1 NZ_CP018241:1109735-1110112 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTCSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGVKQ

Nucleotide

• Length: 378 bp

>T69058 NZ_CP018241:1109735-1110112 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCTGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13578.33 Da
• Isoelectric Point: 6.7384

>AT69058 WP_033805992.1 NZ_CP018241:1109277-1109645 [Escherichia coli]
VSDTLHETNHPDGNNNSPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGQFSDADVYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSKTKK

Nucleotide

• Length: 369 bp

>AT69058 NZ_CP018241:1109277-1109645 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATCACCCCGACGGCAATAACAACAGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGTATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCAAAACGAAAAAGTAA