Detailed information of TA system TA069025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2135153..2135624
• Replicon: chromosome
• Accession: NZ_CP018239
• Organism: Escherichia coli strain 272

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: BFL22_RS11775
• Protein ID: WP_001303511.1
• Coordinates: 2135346..2135624 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: BFL22_RS11770
• Protein ID: WP_001302048.1
• Coordinates: 2135153..2135344 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL22_RS11725	2130360..2130944	-	585	WP_000206793.1	DUF551 domain-containing protein	-
BFL22_RS11730	2131001..2131396	-	396	WP_001118159.1	DUF977 family protein	-
BFL22_RS11735	2131412..2132181	-	770	Protein_2066	DUF1627 domain-containing protein	-
BFL22_RS11745	2132207..2132947	-	741	WP_000788938.1	ATP-binding protein	-
BFL22_RS11750	2132954..2133916	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
BFL22_RS11755	2133939..2134364	-	426	WP_000693943.1	Rha family transcriptional regulator	-
BFL22_RS11760	2134361..2134663	-	303	WP_000172738.1	transcriptional regulator	-
BFL22_RS11765	2134761..2135132	+	372	WP_001169686.1	hypothetical protein	-
BFL22_RS11770	2135153..2135344	+	192	WP_001302048.1	hypothetical protein	Antitoxin
BFL22_RS11775	2135346..2135624	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFL22_RS11785	2135976..2136275	+	300	WP_001240334.1	hypothetical protein	-
BFL22_RS11790	2136347..2136565	+	219	WP_001171930.1	hypothetical protein	-
BFL22_RS11800	2137134..2137322	+	189	WP_000449192.1	cell division inhibition protein DicB	-
BFL22_RS11805	2137319..2137510	+	192	WP_001090200.1	DUF1482 family protein	-
BFL22_RS11810	2137603..2140074	+	2472	WP_000048458.1	exonuclease	-
BFL22_RS11815	2140147..2140398	+	252	WP_000005552.1	excisionase family protein	-
BFL22_RS31505	2140433..2140617	+	185	Protein_2080	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2045750..2143910	98160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T69025 WP_001303511.1 NZ_CP018239:2135346-2135624 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T69025 NZ_CP018239:2135346-2135624 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT69025 WP_001302048.1 NZ_CP018239:2135153-2135344 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT69025 NZ_CP018239:2135153-2135344 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0