Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 70578..71137 Replicon plasmid pO157
Accession NZ_CP018238
Organism Escherichia coli strain 155

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag BFL20_RS30635 Protein ID WP_000421248.1
Coordinates 70862..71137 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag BFL20_RS30630 Protein ID WP_001178089.1
Coordinates 70578..70862 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL20_RS30575 65769..66329 + 561 WP_000840472.1 fertility inhibition protein FinO -
BFL20_RS30580 66462..66722 + 261 WP_072612820.1 ANR family transcriptional regulator -
BFL20_RS30585 66920..67381 + 462 WP_001233853.1 thermonuclease family protein -
BFL20_RS30590 67427..67636 + 210 WP_001302200.1 hemolysin expression modulator Hha -
BFL20_RS30595 67674..68012 + 339 WP_000766796.1 hypothetical protein -
BFL20_RS30600 68252..68506 + 255 WP_000083831.1 replication regulatory protein RepA -
BFL20_RS30610 68742..68816 + 75 WP_001370046.1 RepA leader peptide Tap -
BFL20_RS30615 68809..69666 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
BFL20_RS30630 70578..70862 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
BFL20_RS30635 70862..71137 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BFL20_RS30640 71232..71438 + 207 WP_001105064.1 hypothetical protein -
BFL20_RS30650 71538..71753 - 216 Protein_77 transposase -
BFL20_RS30655 71797..72363 - 567 Protein_78 IS3-like element IS600 family transposase -
BFL20_RS30660 72363..72779 - 417 Protein_79 IS91 family transposase -
BFL20_RS30665 72779..72964 - 186 WP_001302179.1 hypothetical protein -
BFL20_RS30670 73141..75351 + 2211 WP_000592771.1 catalase/peroxidase KatP -
BFL20_RS30675 75395..75784 + 390 WP_001172748.1 cytochrome b562 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP / espP 1..91420 91420


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T69010 WP_000421248.1 NZ_CP018238:70862-71137 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T69010 NZ_CP018238:70862-71137 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT69010 WP_001178089.1 NZ_CP018238:70578-70862 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT69010 NZ_CP018238:70578-70862 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References