Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4391022..4391653 Replicon chromosome
Accession NZ_CP018237
Organism Escherichia coli strain 155

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag BFL20_RS24445 Protein ID WP_001259386.1
Coordinates 4391378..4391653 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BFL20_RS24440 Protein ID WP_000593555.1
Coordinates 4391022..4391381 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL20_RS24410 4386134..4386607 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
BFL20_RS24415 4386604..4386885 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
BFL20_RS24420 4386962..4388320 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
BFL20_RS24425 4388313..4389821 + 1509 WP_001301773.1 carbohydrate kinase -
BFL20_RS24430 4389811..4390080 + 270 WP_000933060.1 HPr family phosphocarrier protein -
BFL20_RS24435 4390112..4390972 + 861 WP_001131788.1 class II aldolase -
BFL20_RS24440 4391022..4391381 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BFL20_RS24445 4391378..4391653 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
BFL20_RS24450 4391726..4392850 - 1125 WP_001301659.1 ABC transporter permease -
BFL20_RS24455 4392850..4395585 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
BFL20_RS24460 4395582..4396649 - 1068 WP_072612792.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T69000 WP_001259386.1 NZ_CP018237:c4391653-4391378 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T69000 NZ_CP018237:c4391653-4391378 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT69000 WP_000593555.1 NZ_CP018237:c4391381-4391022 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT69000 NZ_CP018237:c4391381-4391022 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References