Detailed information of TA system TA068952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2599996..2600669
• Replicon: chromosome
• Accession: NZ_CP018221
• Organism: Tardibacter chloracetimidivorans strain JJ-A5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1E1F821
• Locus tag: BSL82_RS13455
• Protein ID: WP_048574953.1
• Coordinates: 2599996..2600409 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0F7JTK4
• Locus tag: BSL82_RS13460
• Protein ID: WP_047100244.1
• Coordinates: 2600406..2600669 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSL82_RS13435	2595484..2596461	-	978	WP_048574804.1	DUF2493 domain-containing protein	-
BSL82_RS13440	2596941..2598065	+	1125	WP_066518892.1	hypothetical protein	-
BSL82_RS13445	2598115..2598435	-	321	WP_042469252.1	single-stranded DNA-binding protein	-
BSL82_RS13450	2598697..2599899	-	1203	WP_048574954.1	SEC-C domain-containing protein	-
BSL82_RS13455	2599996..2600409	-	414	WP_048574953.1	type II toxin-antitoxin system VapC family toxin	Toxin
BSL82_RS13460	2600406..2600669	-	264	WP_047100244.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BSL82_RS13465	2600857..2601360	-	504	WP_030541254.1	DUF736 domain-containing protein	-
BSL82_RS13470	2601530..2602093	-	564	WP_081856824.1	MucR family transcriptional regulator	-
BSL82_RS13475	2602269..2602583	-	315	WP_030541252.1	hypothetical protein	-
BSL82_RS13480	2602643..2603341	-	699	WP_048574952.1	hypothetical protein	-
BSL82_RS13485	2603331..2603819	-	489	WP_030541250.1	hypothetical protein	-
BSL82_RS13490	2603961..2604290	-	330	WP_048574951.1	hypothetical protein	-
BSL82_RS13495	2604287..2604481	-	195	WP_030541248.1	hypothetical protein	-
BSL82_RS13500	2604616..2604948	-	333	WP_030541247.1	DUF3768 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2469980..2620391	150411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (16-53)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14415.57 Da
• Isoelectric Point: 5.6141

>T68952 WP_048574953.1 NZ_CP018221:c2600409-2599996 [Tardibacter chloracetimidivorans]
VTQLYMLDTNIVSELARNPHGAVATRIAQVGADAICVSIITAAELRYGCAKKGSPRLLAQMEAILGSVSVLALDVPADTE
YGGIRAELESAGKPIGPNDLFIAAHAYALGATLVTANIGEFSRVRALKVENWLSDVH

Nucleotide

• Length: 414 bp

>T68952 NZ_CP018221:c2600409-2599996 [Tardibacter chloracetimidivorans]
GTGACCCAGCTCTACATGCTCGATACCAACATCGTGTCGGAACTCGCGCGAAACCCGCACGGCGCCGTAGCCACCCGGAT
CGCGCAAGTCGGTGCCGATGCGATTTGCGTCAGCATCATCACCGCGGCCGAGCTGCGCTACGGCTGCGCGAAGAAGGGGT
CCCCCAGGCTCCTGGCCCAAATGGAGGCGATCCTCGGCAGCGTGTCCGTGCTTGCGCTCGATGTCCCGGCGGACACTGAA
TATGGCGGTATCCGAGCCGAGCTGGAGAGCGCGGGCAAACCCATCGGGCCCAACGACCTCTTCATCGCGGCTCATGCCTA
TGCGCTCGGCGCCACATTGGTGACCGCCAACATCGGCGAGTTCTCCCGCGTCCGGGCCCTCAAGGTGGAGAATTGGCTGA
GCGACGTCCATTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9838.22 Da
• Isoelectric Point: 4.7010

>AT68952 WP_047100244.1 NZ_CP018221:c2600669-2600406 [Tardibacter chloracetimidivorans]
VTPDHEATVTREAKLFRNNKSQAVRIPADFELPGDRVMIHREGDRLVIEPIRRRNIVEVLASLEPLGPEDEFPDIDSTLL
PAKAIEL

Nucleotide

• Length: 264 bp

>AT68952 NZ_CP018221:c2600669-2600406 [Tardibacter chloracetimidivorans]
GTGACGCCCGATCATGAAGCAACGGTCACCAGAGAAGCGAAGCTGTTCCGCAACAACAAGAGCCAGGCAGTGCGCATCCC
GGCCGATTTCGAGCTGCCTGGAGATCGGGTCATGATCCACCGGGAGGGAGATCGACTGGTCATCGAGCCGATCCGCCGCC
GAAACATCGTTGAGGTCCTTGCGAGCCTCGAACCCCTTGGCCCCGAGGATGAATTCCCGGACATCGACAGCACCTTGCTT
CCGGCCAAGGCCATCGAATTGTGA

Structures

Toxin

• AlphaFold DB: A0A1E1F821

Antitoxin

• AlphaFold DB: A0A0F7JTK4