Detailed information of TA system TA068947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 53703..54304
• Replicon: plasmid pMRSN346647_113.1
• Accession: NZ_CP018207
• Organism: Escherichia coli strain MRSN346647

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: BSZ13_RS26500
• Protein ID: WP_001216034.1
• Coordinates: 53924..54304 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: BSZ13_RS26495
• Protein ID: WP_001190712.1
• Coordinates: 53703..53924 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSZ13_RS26440	49851..50465	-	615	WP_032214276.1	acid-sensing system DNA-binding response regulator EvgA	-
BSZ13_RS27410	50839..51536	+	698	Protein_51	IS1 family transposase	-
BSZ13_RS26465	51855..51959	+	105	Protein_52	IS3 family transposase	-
BSZ13_RS26470	52299..52556	+	258	WP_072617411.1	biofilm development YmgB/AriR family protein	-
BSZ13_RS26475	52743..53440	-	698	Protein_54	IS1-like element IS1A family transposase	-
BSZ13_RS26495	53703..53924	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BSZ13_RS26500	53924..54304	+	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
BSZ13_RS26505	54309..54488	+	180	WP_001513661.1	hypothetical protein	-
BSZ13_RS26510	54516..54875	+	360	WP_072617413.1	pdcB	-
BSZ13_RS26515	54799..55212	+	414	Protein_59	DDE-type integrase/transposase/recombinase	-
BSZ13_RS26520	55162..55479	-	318	WP_001513659.1	hypothetical protein	-
BSZ13_RS26525	55707..56687	-	981	Protein_61	IS5 family transposase	-
BSZ13_RS26530	56931..58334	+	1404	WP_001373486.1	S-methylmethionine permease	-
BSZ13_RS26535	58321..59253	+	933	WP_000081352.1	homocysteine S-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / blaCTX-M-15 / qnrS1	-	1..113140	113140
	-	inside	IScluster/Tn	-	-	51141..61287	10146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T68947 WP_001216034.1 NZ_CP018207:53924-54304 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T68947 NZ_CP018207:53924-54304 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT68947 WP_001190712.1 NZ_CP018207:53703-53924 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT68947 NZ_CP018207:53703-53924 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6