Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3862569..3863223 Replicon chromosome
Accession NZ_CP018206
Organism Escherichia coli strain MRSN346647

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag BSZ13_RS20075 Protein ID WP_000244781.1
Coordinates 3862569..3862976 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BSZ13_RS20080 Protein ID WP_000354046.1
Coordinates 3862957..3863223 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSZ13_RS20055 3858526..3860259 - 1734 WP_001598594.1 single-stranded-DNA-specific exonuclease RecJ -
BSZ13_RS20060 3860265..3860975 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BSZ13_RS20065 3861000..3861896 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
BSZ13_RS20070 3862008..3862529 + 522 WP_001055874.1 flavodoxin FldB -
BSZ13_RS20075 3862569..3862976 - 408 WP_000244781.1 protein YgfX Toxin
BSZ13_RS20080 3862957..3863223 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BSZ13_RS20085 3863466..3864446 + 981 WP_001562036.1 tRNA-modifying protein YgfZ -
BSZ13_RS20090 3864523..3865182 - 660 WP_000250269.1 hemolysin III family protein -
BSZ13_RS20095 3865346..3865657 - 312 WP_001598596.1 N(4)-acetylcytidine aminohydrolase -
BSZ13_RS20100 3865702..3867135 + 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T68944 WP_000244781.1 NZ_CP018206:c3862976-3862569 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T68944 NZ_CP018206:c3862976-3862569 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT68944 WP_000354046.1 NZ_CP018206:c3863223-3862957 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT68944 NZ_CP018206:c3863223-3862957 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References