Detailed information of TA system TA068930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2194755..2195393
• Replicon: chromosome
• Accession: NZ_CP018206
• Organism: Escherichia coli strain MRSN346647

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: BSZ13_RS11275
• Protein ID: WP_000813795.1
• Coordinates: 2194755..2194931 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BSZ13_RS11280
• Protein ID: WP_076797675.1
• Coordinates: 2194977..2195393 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSZ13_RS11255	2190377..2191588	-	1212	WP_071591517.1	BenE family transporter YdcO	-
BSZ13_RS11260	2191641..2192177	+	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
BSZ13_RS11265	2192250..2194211	+	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
BSZ13_RS11270	2194303..2194533	-	231	WP_023910283.1	YncJ family protein	-
BSZ13_RS11275	2194755..2194931	+	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
BSZ13_RS11280	2194977..2195393	+	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
BSZ13_RS11285	2195472..2196878	+	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
BSZ13_RS11290	2197123..2198268	+	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
BSZ13_RS11295	2198286..2199299	+	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
BSZ13_RS11300	2199300..2200241	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T68930 WP_000813795.1 NZ_CP018206:2194755-2194931 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T68930 NZ_CP018206:2194755-2194931 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT68930 WP_076797675.1 NZ_CP018206:2194977-2195393 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT68930 NZ_CP018206:2194977-2195393 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1