Detailed information of TA system TA068923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1515514..1516219
• Replicon: chromosome
• Accession: NZ_CP018206
• Organism: Escherichia coli strain MRSN346647

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: BSZ13_RS07660
• Protein ID: WP_000539521.1
• Coordinates: 1515833..1516219 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BSZ13_RS07655
• Protein ID: WP_001280945.1
• Coordinates: 1515514..1515843 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSZ13_RS07640	1510671..1511582	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
BSZ13_RS07645	1511760..1514108	+	2349	WP_001328220.1	EAL domain-containing protein	-
BSZ13_RS07650	1514116..1515444	+	1329	WP_049068068.1	GGDEF domain-containing protein	-
BSZ13_RS07655	1515514..1515843	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
BSZ13_RS07660	1515833..1516219	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BSZ13_RS07665	1516445..1517770	-	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
BSZ13_RS07670	1517983..1518366	+	384	WP_000497137.1	biofilm formation regulator BssR	-
BSZ13_RS07675	1518477..1519592	+	1116	WP_001595547.1	aldose sugar dehydrogenase YliI	-
BSZ13_RS07680	1519589..1520215	-	627	WP_001595548.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T68923 WP_000539521.1 NZ_CP018206:c1516219-1515833 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T68923 NZ_CP018206:c1516219-1515833 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT68923 WP_001280945.1 NZ_CP018206:c1515843-1515514 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT68923 NZ_CP018206:c1515843-1515514 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6