Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1056593..1057211 Replicon chromosome
Accession NZ_CP018206
Organism Escherichia coli strain MRSN346647

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BSZ13_RS05335 Protein ID WP_001291435.1
Coordinates 1056593..1056811 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BSZ13_RS05340 Protein ID WP_000344800.1
Coordinates 1056837..1057211 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSZ13_RS05300 1051882..1052454 + 573 WP_000779831.1 YbaY family lipoprotein -
BSZ13_RS05305 1052485..1052796 - 312 WP_000409911.1 MGMT family protein -
BSZ13_RS05315 1053175..1053528 + 354 WP_001550741.1 DUF1428 family protein -
BSZ13_RS05320 1053570..1055120 - 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BSZ13_RS05325 1055284..1055754 - 471 WP_000136192.1 YlaC family protein -
BSZ13_RS05330 1055870..1056421 - 552 WP_000102564.1 maltose O-acetyltransferase -
BSZ13_RS05335 1056593..1056811 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BSZ13_RS05340 1056837..1057211 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BSZ13_RS05345 1057757..1060906 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
BSZ13_RS05350 1060929..1062122 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T68922 WP_001291435.1 NZ_CP018206:c1056811-1056593 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T68922 NZ_CP018206:c1056811-1056593 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT68922 WP_000344800.1 NZ_CP018206:c1057211-1056837 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT68922 NZ_CP018206:c1057211-1056837 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References