Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/StbC(antitoxin)
Location 5912058..5912458 Replicon chromosome
Accession NZ_CP018202
Organism Pseudomonas syringae pv. actinidiae ICMP 9853

Toxin (Protein)


Gene name vapC Uniprot ID A0A2V0QK73
Locus tag JN853_RS28520 Protein ID WP_003381344.1
Coordinates 5912312..5912458 (+) Length 49 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag JN853_RS28515 Protein ID WP_003381346.1
Coordinates 5912058..5912315 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN853_RS28485 5908224..5908412 - 189 Protein_5218 RHS repeat-associated core domain-containing protein -
JN853_RS32410 5908463..5910042 + 1580 WP_085991147.1 IS3-like element ISPsy31 family transposase -
JN853_RS28505 5910737..5910991 - 255 WP_003381350.1 hypothetical protein -
JN853_RS28510 5911039..5911695 - 657 WP_017684244.1 hypothetical protein -
JN853_RS28515 5912058..5912315 + 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
JN853_RS28520 5912312..5912458 + 147 WP_003381344.1 hypothetical protein Toxin
JN853_RS28525 5912753..5913688 + 936 WP_003381342.1 syringolide biosynthetic protein AvrD1 -
JN853_RS28530 5914029..5914676 + 648 WP_003381340.1 histidine phosphatase family protein -
JN853_RS28535 5914822..5915742 + 921 WP_005620530.1 TauD/TfdA family dioxygenase -
JN853_RS28540 5915760..5916794 + 1035 WP_003381338.1 sulfotransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5910737..5929595 18858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 49 a.a.        Molecular weight: 5447.25 Da        Isoelectric Point: 4.7333

>T68895 WP_003381344.1 NZ_CP018202:5912312-5912458 [Pseudomonas syringae pv. actinidiae ICMP 9853]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLAGVSVSQVKRVWA

Download         Length: 147 bp

>T68895 NZ_CP018202:5912312-5912458 [Pseudomonas syringae pv. actinidiae ICMP 9853]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATCTGGCCGGTGTCAGTGTCAGCCAGGTAAAGCGTGTCTGGGCATAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT68895 WP_003381346.1 NZ_CP018202:5912058-5912315 [Pseudomonas syringae pv. actinidiae ICMP 9853]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT68895 NZ_CP018202:5912058-5912315 [Pseudomonas syringae pv. actinidiae ICMP 9853]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QK73

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References