Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 590423..592152 Replicon chromosome
Accession NZ_CP018202
Organism Pseudomonas syringae pv. actinidiae ICMP 9853

Toxin (Protein)


Gene name pmenT Uniprot ID A0A2V0QPP4
Locus tag JN853_RS03080 Protein ID WP_003379114.1
Coordinates 591508..592152 (-) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A2V0QNZ0
Locus tag JN853_RS03075 Protein ID WP_003379115.1
Coordinates 590423..591511 (-) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN853_RS03050 585974..586750 + 777 WP_003379118.1 ABC transporter permease -
JN853_RS03055 586747..586998 + 252 WP_017699326.1 hypothetical protein -
JN853_RS03060 587079..588593 + 1515 WP_077142728.1 IS21-like element ISPsy41 family transposase -
JN853_RS03065 588614..589369 + 756 WP_017699032.1 IS21-like element ISPsy14 family helper ATPase IstB -
JN853_RS03070 589474..589989 + 516 Protein_561 ABC transporter ATP-binding protein -
JN853_RS33715 590070..590323 + 254 Protein_562 glycosyl transferase family 2 -
JN853_RS03075 590423..591511 - 1089 WP_003379115.1 macro domain-containing protein Antitoxin
JN853_RS03080 591508..592152 - 645 WP_003379114.1 DUF4433 domain-containing protein Toxin
JN853_RS03085 592149..592730 - 582 WP_003379113.1 DUF4433 domain-containing protein -
JN853_RS03090 593148..594269 + 1122 WP_003379112.1 DUF91 domain-containing protein -
JN853_RS03105 595863..596594 - 732 WP_003379109.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 582668..595539 12871


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24813.38 Da        Isoelectric Point: 6.4920

>T68890 WP_003379114.1 NZ_CP018202:c592152-591508 [Pseudomonas syringae pv. actinidiae ICMP 9853]
MIYSSLNPEKALIWRIVHRDNLPWILDNGLHCASSYMQAPQYVNIGNADLIDKRRNRSVPIAPAGVLSDYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLPRIEQLGLAFVFTNAHAYPDWTDYYSDLVNLDQIDWPIIQKRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYDDAMKERIEQDVAARGLTLPVHARPGWYFQ

Download         Length: 645 bp

>T68890 NZ_CP018202:c592152-591508 [Pseudomonas syringae pv. actinidiae ICMP 9853]
ATGATCTACAGCAGTCTGAATCCAGAAAAAGCTCTGATTTGGCGAATCGTCCACCGCGATAATCTGCCTTGGATTCTGGA
CAATGGCCTGCATTGCGCAAGCTCCTACATGCAAGCACCGCAGTACGTGAACATCGGCAACGCTGACCTGATCGACAAAC
GCCGCAATCGCAGTGTTCCAATTGCTCCAGCGGGCGTACTGTCCGACTACGTGCCCTTCTACTTCACACCTTTCTCTGTG
ATGATGAAAAATATCCATTCCGGCTGGAGCGTTCAGCAGCGTAGCAATGACGAGATAGTGATTCTGGTCTCAAGCCTGCC
TCGTATTGAGCAGCTTGGCCTGGCGTTCGTCTTTACCAACGCACACGCCTACCCTGACTGGACCGATTACTACAGCGATC
TGGTGAATCTCGACCAGATCGACTGGCCTATAATCCAGAAACGAGACTTCAAGCGTGACCCTGATGATCCGCGTAAGATG
GAGCGTTATCAGGCCGAAGCGCTCATTCACCATCACCTACCGATTACAGGACTCCTGGGCATCATGTGCTACGACGATGC
AATGAAAGAACGCATTGAGCAAGACGTTGCCGCCCGAGGTCTGACTTTGCCTGTCCACGCGCGTCCGGGATGGTATTTCC
AATGA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40646.66 Da        Isoelectric Point: 6.1829

>AT68890 WP_003379115.1 NZ_CP018202:c591511-590423 [Pseudomonas syringae pv. actinidiae ICMP 9853]
MIRFTQGNLLEADTEALVNTVNTVGVMGKGIALMFKERFTENYRLYSAACKAGEVETGKVHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLRDLRRFLIDNHVKSVAVPPLGAGNGGLKWVEVREQIVDVLSDLDVDVLVFEPTDQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIEQLPSAKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGIDDVIWFDEGRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLCKSGVSPTVPTVREA
LKHWDGGAGAAARKSRLFDDKAIDIALKRLTTSSLSPEMPPS

Download         Length: 1089 bp

>AT68890 NZ_CP018202:c591511-590423 [Pseudomonas syringae pv. actinidiae ICMP 9853]
ATGATCAGATTCACCCAAGGCAATCTGCTGGAAGCAGATACCGAAGCTCTCGTCAACACGGTGAACACTGTAGGCGTAAT
GGGAAAAGGCATTGCGCTGATGTTCAAGGAGCGCTTTACCGAAAACTATCGCCTCTATTCTGCGGCGTGCAAGGCGGGTG
AGGTAGAGACCGGAAAGGTTCACGTAACGGCAGTCAACGAGCTGGACGGCCCGCGCTGGATAGTGAATTTCCCTACCAAG
CGTCACTGGCGTTCCCCCTCGCAGATGGCATGGATCACCGAAGGCTTACGGGATCTGCGCCGTTTTTTGATCGATAACCA
CGTGAAGTCCGTAGCGGTTCCACCTCTCGGGGCGGGTAATGGCGGTTTGAAATGGGTAGAGGTCCGTGAGCAGATTGTTG
ATGTCTTGAGTGATCTGGATGTCGATGTACTTGTCTTTGAACCCACTGATCAATATCTGAATGTCGCGAAGCGTAGCGGT
GTCGAGAAACTGACGCCTGCACGCGCACTCATTGCCGAACTCGTTCGGCGTTACTGGGTTTTGGGCATGGAATGCAGCCT
GCTTGAGATTCAGAAGCTCGCTTGGTTTCTGGAGCGTTCGATAGAGCAATTGCCAAGCGCCAAGAACCCCTTGGATCTCA
AATTTGTAGCCCACAAATATGGGCCATACGCCAATCGCCTTGAGCATCTGCTCGACAATCTCGATGGTAGCTACCTGCAT
TGCGATAAACGCATCAGCGACGCAGGTATTGACGACGTGATCTGGTTTGACGAGGGCCGTAAGGCTTTTCTGCAAACTTA
TCTGAAAACAGAGGCCAAGGAATATTCCCAGGCTCTGGAGCGCACCGCAGAGTTGATTGACGGCTTTGAGTCGCCTTTCG
GCATGGAACTGTTGGCGACGGTTGACTGGTTATTGTGTAAGTCGGGAGTGTCTCCAACGGTTCCGACAGTGCGTGAAGCA
TTGAAGCATTGGGATGGAGGCGCAGGTGCGGCAGCTCGGAAAAGCAGGTTGTTTGATGATAAAGCGATCGACATTGCTCT
AAAGCGATTGACTACCAGTAGTCTCAGCCCAGAGATGCCCCCTTCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPP4

Antitoxin

Source ID Structure
AlphaFold DB A0A2V0QNZ0

References