Detailed information of TA system TA068888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1986310..1986947
• Replicon: chromosome
• Accession: NZ_CP018200
• Organism: Bacillus amyloliquefaciens strain WS-8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BSO20_RS09420
• Protein ID: WP_003156187.1
• Coordinates: 1986310..1986660 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BSO20_RS09425
• Protein ID: WP_003156188.1
• Coordinates: 1986666..1986947 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSO20_RS09380	1981350..1981952	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
BSO20_RS09385	1981952..1982740	-	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
BSO20_RS09390	1982706..1983188	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
BSO20_RS09395	1983185..1983514	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BSO20_RS09400	1983578..1984585	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
BSO20_RS09405	1984597..1984998	-	402	WP_003156178.1	anti-sigma regulatory factor	-
BSO20_RS09410	1985001..1985366	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BSO20_RS09415	1985371..1986192	-	822	WP_003156182.1	STAS domain-containing protein	-
BSO20_RS09420	1986310..1986660	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BSO20_RS09425	1986666..1986947	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BSO20_RS09430	1987067..1988236	-	1170	WP_032873001.1	alanine racemase	-
BSO20_RS09435	1988353..1989360	-	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
BSO20_RS09440	1989525..1989890	-	366	WP_007609580.1	holo-ACP synthase	-
BSO20_RS09445	1989983..1990582	+	600	WP_032872997.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T68888 WP_003156187.1 NZ_CP018200:c1986660-1986310 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T68888 NZ_CP018200:c1986660-1986310 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT68888 WP_003156188.1 NZ_CP018200:c1986947-1986666 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT68888 NZ_CP018200:c1986947-1986666 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5