Detailed information of TA system TA068887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1234791..1235708
• Replicon: chromosome
• Accession: NZ_CP018200
• Organism: Bacillus amyloliquefaciens strain WS-8

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: BSO20_RS05440
• Protein ID: WP_007407256.1
• Coordinates: 1234791..1235537 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: BSO20_RS05445
• Protein ID: WP_003154807.1
• Coordinates: 1235538..1235708 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSO20_RS05420	1230184..1231134	-	951	WP_032874618.1	ring-cleaving dioxygenase	-
BSO20_RS05425	1231456..1232772	+	1317	WP_032874616.1	amino acid permease	-
BSO20_RS05430	1233058..1233675	+	618	WP_032874614.1	DUF47 domain-containing protein	-
BSO20_RS05435	1233688..1234686	+	999	WP_032874611.1	inorganic phosphate transporter	-
BSO20_RS05440	1234791..1235537	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BSO20_RS05445	1235538..1235708	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BSO20_RS05450	1235965..1236843	-	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
BSO20_RS05455	1236857..1237120	-	264	WP_003154813.1	phage holin	-
BSO20_RS05460	1237134..1237397	-	264	WP_003154815.1	hemolysin XhlA family protein	-
BSO20_RS05465	1237449..1238210	-	762	WP_032874607.1	hypothetical protein	-
BSO20_RS05470	1238267..1238464	-	198	WP_032874605.1	XkdX family protein	-
BSO20_RS05475	1238469..1238840	-	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
BSO20_RS05480	1238853..1240475	-	1623	WP_032874601.1	pyocin knob domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1220424..1282646	62222

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T68887 WP_007407256.1 NZ_CP018200:1234791-1235537 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T68887 NZ_CP018200:1234791-1235537 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT68887 WP_003154807.1 NZ_CP018200:1235538-1235708 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT68887 NZ_CP018200:1235538-1235708 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14