Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 520782..521418 Replicon chromosome
Accession NZ_CP018197
Organism Bacillus safensis strain KCTC 12796BP

Toxin (Protein)


Gene name mazF Uniprot ID A0A0P7G713
Locus tag BSL056_RS02505 Protein ID WP_024425388.1
Coordinates 521068..521418 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag BSL056_RS02500 Protein ID WP_003214273.1
Coordinates 520782..521063 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSL056_RS02480 516922..517527 - 606 WP_024425384.1 rhomboid family intramembrane serine protease -
BSL056_RS02485 517622..517987 + 366 WP_047202005.1 holo-ACP synthase -
BSL056_RS02490 518148..519164 + 1017 WP_087975235.1 outer membrane lipoprotein carrier protein LolA -
BSL056_RS02495 519306..520487 + 1182 WP_044334720.1 alanine racemase -
BSL056_RS02500 520782..521063 + 282 WP_003214273.1 hypothetical protein Antitoxin
BSL056_RS02505 521068..521418 + 351 WP_024425388.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BSL056_RS02510 521535..522365 + 831 WP_044334717.1 RsbT co-antagonist protein RsbRA -
BSL056_RS02515 522370..522738 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
BSL056_RS02520 522741..523142 + 402 WP_073203956.1 anti-sigma regulatory factor -
BSL056_RS02525 523153..524160 + 1008 WP_024425391.1 PP2C family protein-serine/threonine phosphatase -
BSL056_RS02530 524220..524549 + 330 WP_024425392.1 anti-sigma factor antagonist -
BSL056_RS02535 524546..525034 + 489 WP_024425393.1 anti-sigma B factor RsbW -
BSL056_RS02540 525000..525788 + 789 WP_024425394.1 RNA polymerase sigma factor SigB -
BSL056_RS02545 525788..526387 + 600 WP_024425395.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8891

>T68885 WP_024425388.1 NZ_CP018197:521068-521418 [Bacillus safensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T68885 NZ_CP018197:521068-521418 [Bacillus safensis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTGATGCAAAGCGCTACGGCTTTGAGCGAGATTCTGTCATTCTCTTGGAGCAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT68885 WP_003214273.1 NZ_CP018197:520782-521063 [Bacillus safensis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT68885 NZ_CP018197:520782-521063 [Bacillus safensis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTACCGGAGGCTTTGATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P7G713

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References