Detailed information of TA system TA068873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3893381..3894017
• Replicon: chromosome
• Accession: NZ_CP018184
• Organism: Bacillus subtilis strain KH2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BSR08_RS20815
• Protein ID: WP_003156187.1
• Coordinates: 3893667..3894017 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: BSR08_RS20810
• Protein ID: WP_003225183.1
• Coordinates: 3893381..3893662 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSR08_RS20790	3889740..3890339	-	600	WP_014478896.1	rhomboid family intramembrane serine protease	-
BSR08_RS20795	3890434..3890799	+	366	WP_014478897.1	holo-ACP synthase	-
BSR08_RS20800	3890965..3891981	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
BSR08_RS20805	3892096..3893265	+	1170	WP_014478898.1	alanine racemase	-
BSR08_RS20810	3893381..3893662	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BSR08_RS20815	3893667..3894017	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BSR08_RS20820	3894133..3894957	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
BSR08_RS20825	3894962..3895327	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
BSR08_RS20830	3895331..3895732	+	402	WP_014478899.1	serine/threonine-protein kinase RsbT	-
BSR08_RS20835	3895744..3896751	+	1008	WP_014478900.1	phosphoserine phosphatase RsbU	-
BSR08_RS20840	3896820..3897149	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
BSR08_RS20845	3897146..3897628	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
BSR08_RS20850	3897594..3898382	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
BSR08_RS20855	3898382..3898981	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T68873 WP_003156187.1 NZ_CP018184:3893667-3894017 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T68873 NZ_CP018184:3893667-3894017 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT68873 WP_003225183.1 NZ_CP018184:3893381-3893662 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT68873 NZ_CP018184:3893381-3893662 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32