Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 94328..95013 Replicon plasmid unnamed1
Accession NZ_CP018172
Organism Mesorhizobium oceanicum strain B7

Toxin (Protein)


Gene name vapC Uniprot ID A0A1L3SZR4
Locus tag BSQ44_RS25870 Protein ID WP_072608373.1
Coordinates 94328..94750 (-) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1L3SZU5
Locus tag BSQ44_RS25875 Protein ID WP_072608374.1
Coordinates 94747..95013 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSQ44_RS25850 90077..91282 + 1206 WP_072608370.1 plasmid partitioning protein RepA -
BSQ44_RS25855 91358..92342 + 985 Protein_107 plasmid partitioning protein RepB -
BSQ44_RS25860 92509..93831 + 1323 WP_072608371.1 replication initiation protein RepC -
BSQ44_RS25865 93904..94113 - 210 WP_072608372.1 hypothetical protein -
BSQ44_RS25870 94328..94750 - 423 WP_072608373.1 type II toxin-antitoxin system VapC family toxin Toxin
BSQ44_RS25875 94747..95013 - 267 WP_072608374.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BSQ44_RS25880 95095..96338 - 1244 Protein_112 site-specific integrase -
BSQ44_RS25885 96463..97491 + 1029 WP_072608430.1 DUF1403 family protein -
BSQ44_RS25890 97495..98169 + 675 WP_072608375.1 SMC-Scp complex subunit ScpB -
BSQ44_RS25895 98625..99491 - 867 WP_072608376.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..166078 166078


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-129)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(10-54)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15649.86 Da        Isoelectric Point: 4.9726

>T68869 WP_072608373.1 NZ_CP018172:c94750-94328 [Mesorhizobium oceanicum]
VNVLLDTNVLSEVTKPAPDPRVLGWLDQLDEDHSFISVVSIAEIRRGVALMDAGRKREALAEWLARDLPQRFEHRVLHVD
ETIALVWGDLMALAKRHGRGLSSMDGLIAATATAKQLTLATRNTKDFEGFDFELFDPWTA

Download         Length: 423 bp

>T68869 NZ_CP018172:c94750-94328 [Mesorhizobium oceanicum]
GTGAACGTGTTGCTCGACACGAATGTCCTCTCCGAGGTGACGAAGCCTGCCCCCGACCCAAGAGTCTTAGGATGGCTGGA
CCAACTCGATGAGGACCATTCGTTCATCAGCGTGGTATCGATTGCCGAAATCCGGCGCGGCGTCGCCCTCATGGACGCAG
GGCGGAAACGCGAGGCTCTAGCCGAATGGCTAGCGCGAGATCTCCCACAGCGTTTCGAGCATAGAGTGCTGCATGTTGAC
GAGACGATCGCTCTGGTCTGGGGCGATCTGATGGCGCTGGCGAAGCGGCACGGTCGCGGCCTTTCATCCATGGATGGCCT
GATCGCCGCGACAGCGACGGCGAAACAGCTCACCTTGGCGACCCGCAACACGAAGGATTTCGAGGGTTTTGACTTCGAGC
TATTCGATCCTTGGACTGCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9835.17 Da        Isoelectric Point: 6.3615

>AT68869 WP_072608374.1 NZ_CP018172:c95013-94747 [Mesorhizobium oceanicum]
MLSAPKEDASWTVAGAKARLSEVIERAQSAPQTITRNGKPSVVVVSAEEWQRKTVRKGTLAEFLMDSPMRGAELDLERQR
DEPRDLQL

Download         Length: 267 bp

>AT68869 NZ_CP018172:c95013-94747 [Mesorhizobium oceanicum]
ATGCTATCTGCTCCCAAAGAAGACGCCAGCTGGACGGTCGCCGGAGCAAAGGCACGGCTGTCGGAGGTCATTGAGCGTGC
GCAGTCTGCACCTCAAACAATCACCCGGAACGGTAAGCCAAGCGTGGTTGTCGTCTCGGCCGAGGAATGGCAGCGCAAGA
CGGTGCGAAAGGGCACACTTGCGGAGTTCCTGATGGATTCGCCGATGCGCGGCGCCGAGCTTGACCTCGAGCGTCAGCGC
GACGAACCACGTGATCTGCAGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L3SZR4

Antitoxin

Source ID Structure
AlphaFold DB A0A1L3SZU5

References