Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VapB
Location 2168454..2169040 Replicon chromosome
Accession NZ_CP018171
Organism Mesorhizobium oceanicum strain B7

Toxin (Protein)


Gene name vapC Uniprot ID A0A1L3SQV9
Locus tag BSQ44_RS10740 Protein ID WP_072603911.1
Coordinates 2168648..2169040 (+) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1L3SQU6
Locus tag BSQ44_RS10735 Protein ID WP_072603909.1
Coordinates 2168454..2168651 (+) Length 66 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSQ44_RS10715 2164442..2164891 + 450 WP_072603901.1 DNA polymerase III subunit chi -
BSQ44_RS10720 2165005..2165631 + 627 WP_072603903.1 hypothetical protein -
BSQ44_RS10725 2165668..2166474 - 807 WP_072603905.1 crotonase/enoyl-CoA hydratase family protein -
BSQ44_RS10730 2166471..2168348 - 1878 WP_072603907.1 ABC-F family ATP-binding cassette domain-containing protein -
BSQ44_RS10735 2168454..2168651 + 198 WP_072603909.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
BSQ44_RS10740 2168648..2169040 + 393 WP_072603911.1 PIN domain nuclease Toxin
BSQ44_RS10745 2169069..2169581 - 513 WP_072603913.1 DinB family protein -
BSQ44_RS10750 2169651..2170343 + 693 WP_072603916.1 glutathione S-transferase family protein -
BSQ44_RS10755 2170451..2170873 + 423 WP_072603919.1 nucleoside-diphosphate kinase -
BSQ44_RS10760 2171007..2171420 - 414 WP_072603921.1 hypothetical protein -
BSQ44_RS10765 2171900..2172400 - 501 WP_072603923.1 molybdenum cofactor biosynthesis protein MoaE -
BSQ44_RS10770 2172402..2172653 - 252 WP_072603926.1 molybdopterin converting factor subunit 1 -
BSQ44_RS10775 2172664..2173248 - 585 WP_072603928.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-47)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14274.53 Da        Isoelectric Point: 6.7036

>T68863 WP_072603911.1 NZ_CP018171:2168648-2169040 [Mesorhizobium oceanicum]
VILADTSVWIDYFAGRETSAVARLDAAIEDREIVMGDLILVEVLQGAGSEHKLRQIARKLSPIPCVVLCGPDVAPDAAAN
YRALRRRGVTVRGTIDVIIATWCIRNGAQLLHNDRDFVAMEGHLSLAVWR

Download         Length: 393 bp

>T68863 NZ_CP018171:2168648-2169040 [Mesorhizobium oceanicum]
GTGATCCTCGCCGATACATCCGTGTGGATCGACTATTTCGCCGGGCGCGAAACCAGTGCGGTTGCACGCCTGGACGCGGC
GATCGAGGATCGTGAAATCGTGATGGGCGATCTCATCCTCGTTGAGGTGCTGCAAGGCGCAGGGAGTGAGCACAAGCTTC
GCCAGATCGCTCGCAAGCTGTCTCCGATCCCCTGCGTAGTTCTCTGCGGCCCCGACGTCGCTCCTGACGCAGCGGCGAAC
TATCGCGCGTTACGGCGACGCGGCGTCACGGTGCGCGGCACTATCGACGTGATCATCGCTACGTGGTGCATCAGGAACGG
AGCGCAACTCCTGCACAATGATCGCGATTTCGTCGCCATGGAGGGGCATCTGAGCCTGGCCGTGTGGCGCTGA

Antitoxin


Download         Length: 66 a.a.        Molecular weight: 7593.75 Da        Isoelectric Point: 10.0842

>AT68863 WP_072603909.1 NZ_CP018171:2168454-2168651 [Mesorhizobium oceanicum]
VRTNIEIDEALVRELMALTGAKTKRQVVDEALRDQLKWRKAVKDIRSLRGTVEWEGDLDAMRRDK

Download         Length: 198 bp

>AT68863 NZ_CP018171:2168454-2168651 [Mesorhizobium oceanicum]
GTGCGCACCAATATCGAAATCGATGAGGCACTCGTTCGGGAATTGATGGCTCTTACCGGGGCGAAGACGAAGAGACAGGT
CGTCGACGAAGCTCTGCGTGACCAGCTCAAATGGCGCAAGGCCGTGAAGGATATCAGATCGCTTCGAGGAACCGTCGAGT
GGGAAGGCGACCTCGACGCGATGCGCCGGGACAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L3SQV9

Antitoxin

Source ID Structure
AlphaFold DB A0A1L3SQU6

References