Detailed information of TA system TA068862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 860486..861123
• Replicon: chromosome
• Accession: NZ_CP018152
• Organism: Bacillus amyloliquefaciens strain LM2303

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BSF20_RS04535
• Protein ID: WP_003156187.1
• Coordinates: 860486..860836 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BSF20_RS04540
• Protein ID: WP_003156188.1
• Coordinates: 860842..861123 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSF20_RS04495	855526..856128	-	603	WP_003156170.1	SpoIIE family protein phosphatase	-
BSF20_RS04500	856128..856916	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BSF20_RS04505	856882..857364	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
BSF20_RS04510	857361..857690	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BSF20_RS04515	857754..858761	-	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
BSF20_RS04520	858773..859174	-	402	WP_003156178.1	anti-sigma regulatory factor	-
BSF20_RS04525	859177..859542	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BSF20_RS04530	859547..860368	-	822	WP_014304404.1	STAS domain-containing protein	-
BSF20_RS04535	860486..860836	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BSF20_RS04540	860842..861123	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BSF20_RS04545	861243..862412	-	1170	WP_025853726.1	alanine racemase	-
BSF20_RS04550	862529..863536	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
BSF20_RS04555	863701..864066	-	366	WP_003156192.1	holo-ACP synthase	-
BSF20_RS04560	864159..864758	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T68862 WP_003156187.1 NZ_CP018152:c860836-860486 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T68862 NZ_CP018152:c860836-860486 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT68862 WP_003156188.1 NZ_CP018152:c861123-860842 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT68862 NZ_CP018152:c861123-860842 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5