Detailed information of TA system TA068858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 5997388..5998041
• Replicon: chromosome
• Accession: NZ_CP018145
• Organism: Brevibacillus formosus strain NF2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: J2PLP3
• Locus tag: BP422_RS28715
• Protein ID: WP_005830205.1
• Coordinates: 5997691..5998041 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: J2GZH3
• Locus tag: BP422_RS28710
• Protein ID: WP_007721089.1
• Coordinates: 5997388..5997687 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BP422_RS28680	5992469..5993506	+	1038	WP_088910495.1	outer membrane lipoprotein-sorting protein	-
BP422_RS28695	5994245..5995327	+	1083	WP_088910496.1	hypothetical protein	-
BP422_RS28700	5995512..5995952	+	441	WP_088910497.1	hypothetical protein	-
BP422_RS28705	5995955..5997157	+	1203	WP_088910498.1	alanine racemase	-
BP422_RS28710	5997388..5997687	+	300	WP_007721089.1	CopG family ribbon-helix-helix protein	Antitoxin
BP422_RS28715	5997691..5998041	+	351	WP_005830205.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BP422_RS28720	5998248..5998676	+	429	WP_088910499.1	anti-sigma regulatory factor	-
BP422_RS28725	5998682..5999683	+	1002	WP_088910500.1	PP2C family protein-serine/threonine phosphatase	-
BP422_RS28730	5999707..6000033	+	327	WP_088910501.1	anti-sigma factor antagonist	-
BP422_RS28735	6000033..6000527	+	495	WP_088910502.1	anti-sigma B factor RsbW	-
BP422_RS28740	6000496..6001284	+	789	WP_088910503.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-52)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12917.86 Da
• Isoelectric Point: 4.8435

>T68858 WP_005830205.1 NZ_CP018145:5997691-5998041 [Brevibacillus formosus]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T68858 NZ_CP018145:5997691-5998041 [Brevibacillus formosus]
GTGATTGTCAAACGTGGCGATGTGTTTTTCGCGGACCTGTCCCCGGTCGTTGGCTCTGAGCAAGGAGGCGTACGGCCTGT
TTTAGTCATTCAAAACGACATTGGAAATCGATTTAGTCCGACGGTCATCGTCGCAGCCATTACAGCGCAGATTCAAAAGG
CGAAGCTGCCAACGCATGTGGAAATTGACGCAAAAACTTATGGATTTGATCGTGACTCCGTTATTCTGTTGGAACAAATA
CGAACCATTGACAAACAACGTCTCACTGATAAAATTACGCATCTCGATGACGAGATGATGGATCGGGTTAATGAATCCTT
GCAAATCAGTTTAGGACTCATTGACTTTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11309.19 Da
• Isoelectric Point: 10.2101

>AT68858 WP_007721089.1 NZ_CP018145:5997388-5997687 [Brevibacillus formosus]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLNMASE
AFGAEEEADHTLVRLVSGV

Nucleotide

• Length: 300 bp

>AT68858 NZ_CP018145:5997388-5997687 [Brevibacillus formosus]
ATGGTTGGAGGTGGATTTGTCTTGTCGAAGTCAAACACGAAACGCATCATGATCAGCTTGCCACAACACTTGCTTCAGGA
AGTGGATGGCGTTATTCAAAAGGAAAAATCCAATCGCAGCGAGTTTATTCGTCAAGCGATGCATTTGTATTTGAAAGAGC
GAAAAAAACGTACAATCCGTGAGACCATGCAAAAAGGGTACATGGAGATGGCGAAGATCAATCTGAATATGGCATCGGAG
GCTTTTGGCGCTGAAGAAGAGGCTGACCATACTCTTGTCCGCTTAGTTAGCGGGGTGTGA

Structures

Toxin

• AlphaFold DB: J2PLP3

Antitoxin

• AlphaFold DB: J2GZH3