Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4070596..4071215 Replicon chromosome
Accession NZ_CP018140
Organism Klebsiella pneumoniae strain Kp_Goe_822579

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag BB744_RS21035 Protein ID WP_002892050.1
Coordinates 4070997..4071215 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag BB744_RS21030 Protein ID WP_002892066.1
Coordinates 4070596..4070970 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB744_RS21020 4065748..4066941 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BB744_RS21025 4066964..4070110 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
BB744_RS21030 4070596..4070970 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
BB744_RS21035 4070997..4071215 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
BB744_RS21040 4071378..4071944 + 567 WP_002892030.1 maltose O-acetyltransferase -
BB744_RS32750 4071916..4072056 - 141 WP_004147370.1 hypothetical protein -
BB744_RS21050 4072077..4072547 + 471 WP_020802585.1 YlaC family protein -
BB744_RS21055 4072522..4073973 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
BB744_RS21060 4074074..4074772 + 699 WP_023287311.1 GNAT family N-acetyltransferase -
BB744_RS21065 4074769..4074909 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
BB744_RS21070 4074909..4075172 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T68852 WP_002892050.1 NZ_CP018140:4070997-4071215 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T68852 NZ_CP018140:4070997-4071215 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT68852 WP_002892066.1 NZ_CP018140:4070596-4070970 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT68852 NZ_CP018140:4070596-4070970 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References