Detailed information of TA system TA068842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1474261..1474997
• Replicon: chromosome
• Accession: NZ_CP018140
• Organism: Klebsiella pneumoniae strain Kp_Goe_822579

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: BB744_RS07650
• Protein ID: WP_071836879.1
• Coordinates: 1474515..1474997 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: BB744_RS07645
• Protein ID: WP_003026799.1
• Coordinates: 1474261..1474527 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB744_RS07620	1469907..1471046	+	1140	WP_032433368.1	mannitol dehydrogenase	-
BB744_RS07625	1471075..1471737	+	663	WP_032433366.1	dihydroxyacetone kinase subunit L	-
BB744_RS07630	1471721..1472725	+	1005	WP_032433364.1	dihydroxyacetone kinase subunit DhaK	-
BB744_RS07635	1472743..1473375	+	633	WP_032433362.1	3-hexulose-6-phosphate synthase	-
BB744_RS07640	1473385..1473948	+	564	WP_032433361.1	6-phospho-3-hexuloisomerase	-
BB744_RS07645	1474261..1474527	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
BB744_RS07650	1474515..1474997	+	483	WP_071836879.1	GNAT family N-acetyltransferase	Toxin
BB744_RS07655	1475155..1476687	-	1533	WP_015065592.1	IS3-like element ISKpn38 family transposase	-
BB744_RS07660	1476799..1478202	+	1404	WP_039815096.1	ISNCY-like element ISKpn21 family transposase	-
BB744_RS07665	1478231..1478863	-	633	WP_001567369.1	hypothetical protein	-
BB744_RS07670	1479243..1479842	-	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (80-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17481.06 Da
• Isoelectric Point: 9.8177

>T68842 WP_071836879.1 NZ_CP018140:1474515-1474997 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVYKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T68842 NZ_CP018140:1474515-1474997 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTACAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT68842 WP_003026799.1 NZ_CP018140:1474261-1474527 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT68842 NZ_CP018140:1474261-1474527 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66