Detailed information of TA system TA068830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 514204..514841
• Replicon: chromosome
• Accession: NZ_CP018133
• Organism: Bacillus velezensis strain ATR2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BMJ37_RS02590
• Protein ID: WP_003156187.1
• Coordinates: 514491..514841 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BMJ37_RS02585
• Protein ID: WP_003156188.1
• Coordinates: 514204..514485 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMJ37_RS02565	510569..511168	-	600	WP_029974099.1	rhomboid family intramembrane serine protease	-
BMJ37_RS02570	511261..511626	+	366	WP_007609580.1	holo-ACP synthase	-
BMJ37_RS02575	511791..512798	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
BMJ37_RS02580	512915..514084	+	1170	WP_021495079.1	alanine racemase	-
BMJ37_RS02585	514204..514485	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BMJ37_RS02590	514491..514841	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BMJ37_RS02595	514959..515780	+	822	WP_003156182.1	STAS domain-containing protein	-
BMJ37_RS02600	515785..516150	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
BMJ37_RS02605	516153..516554	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BMJ37_RS02610	516566..517573	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
BMJ37_RS02615	517637..517966	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BMJ37_RS02620	517963..518445	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
BMJ37_RS02625	518411..519199	+	789	WP_029974100.1	RNA polymerase sigma factor SigB	-
BMJ37_RS02630	519199..519801	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T68830 WP_003156187.1 NZ_CP018133:514491-514841 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T68830 NZ_CP018133:514491-514841 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT68830 WP_003156188.1 NZ_CP018133:514204-514485 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT68830 NZ_CP018133:514204-514485 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5