Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 514204..514841 Replicon chromosome
Accession NZ_CP018133
Organism Bacillus velezensis strain ATR2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BMJ37_RS02590 Protein ID WP_003156187.1
Coordinates 514491..514841 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BMJ37_RS02585 Protein ID WP_003156188.1
Coordinates 514204..514485 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BMJ37_RS02565 510569..511168 - 600 WP_029974099.1 rhomboid family intramembrane serine protease -
BMJ37_RS02570 511261..511626 + 366 WP_007609580.1 holo-ACP synthase -
BMJ37_RS02575 511791..512798 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
BMJ37_RS02580 512915..514084 + 1170 WP_021495079.1 alanine racemase -
BMJ37_RS02585 514204..514485 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BMJ37_RS02590 514491..514841 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BMJ37_RS02595 514959..515780 + 822 WP_003156182.1 STAS domain-containing protein -
BMJ37_RS02600 515785..516150 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
BMJ37_RS02605 516153..516554 + 402 WP_003156178.1 anti-sigma regulatory factor -
BMJ37_RS02610 516566..517573 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
BMJ37_RS02615 517637..517966 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BMJ37_RS02620 517963..518445 + 483 WP_007609591.1 anti-sigma B factor RsbW -
BMJ37_RS02625 518411..519199 + 789 WP_029974100.1 RNA polymerase sigma factor SigB -
BMJ37_RS02630 519199..519801 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T68830 WP_003156187.1 NZ_CP018133:514491-514841 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T68830 NZ_CP018133:514491-514841 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT68830 WP_003156188.1 NZ_CP018133:514204-514485 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT68830 NZ_CP018133:514204-514485 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References