Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 456330..456901 Replicon chromosome
Accession NZ_CP018128
Organism Enterococcus faecium strain A_020709_82

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag BSG28_RS02320 Protein ID WP_002286801.1
Coordinates 456560..456901 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag BSG28_RS02315 Protein ID WP_002323011.1
Coordinates 456330..456560 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSG28_RS02265 451466..452794 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
BSG28_RS02270 452816..453442 + 627 WP_002286816.1 cysteine hydrolase -
BSG28_RS02275 453625..454206 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
BSG28_RS02295 454938..455285 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
BSG28_RS02300 455261..455500 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
BSG28_RS02305 455705..456043 - 339 WP_002286804.1 hypothetical protein -
BSG28_RS02315 456330..456560 + 231 WP_002323011.1 hypothetical protein Antitoxin
BSG28_RS02320 456560..456901 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BSG28_RS02325 457751..457936 + 186 WP_002304207.1 hypothetical protein -
BSG28_RS02330 458441..458635 + 195 WP_002297028.1 hypothetical protein -
BSG28_RS02335 458793..459075 + 283 Protein_439 transposase -
BSG28_RS02340 459319..460149 - 831 WP_002286789.1 manganese catalase family protein -
BSG28_RS02345 460357..461691 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T68828 WP_002286801.1 NZ_CP018128:456560-456901 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T68828 NZ_CP018128:456560-456901 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT68828 WP_002323011.1 NZ_CP018128:456330-456560 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT68828 NZ_CP018128:456330-456560 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References