Detailed information of TA system TA068825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 55729..56251
• Replicon: plasmid pMRSN346355_65.5
• Accession: NZ_CP018124
• Organism: Escherichia coli strain MRSN346355

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: BSG24_RS26555
• Protein ID: WP_000638823.1
• Coordinates: 55729..56010 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: BSG24_RS26560
• Protein ID: WP_000121741.1
• Coordinates: 56000..56251 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSG24_RS26520	51878..52198	-	321	WP_000362081.1	VirB3 family type IV secretion system protein	-
BSG24_RS28760	52269..52559	-	291	WP_000865479.1	conjugal transfer protein	-
BSG24_RS26530	52559..53143	-	585	WP_063268637.1	lytic transglycosylase domain-containing protein	-
BSG24_RS26535	53164..53562	-	399	WP_071940825.1	hypothetical protein	-
BSG24_RS26540	53681..54118	-	438	WP_071940826.1	type IV pilus biogenesis protein PilM	-
BSG24_RS26545	54124..55359	-	1236	WP_071940827.1	TcpQ domain-containing protein	-
BSG24_RS26550	55362..55661	-	300	WP_000835764.1	IncN plasmid KikA protein	-
BSG24_RS26555	55729..56010	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BSG24_RS26560	56000..56251	-	252	WP_000121741.1	hypothetical protein	Antitoxin
BSG24_RS26565	56351..56986	-	636	WP_015059536.1	hypothetical protein	-
BSG24_RS26570	57034..57843	+	810	WP_001005148.1	hypothetical protein	-
BSG24_RS26575	58066..58290	-	225	WP_000713561.1	EexN family lipoprotein	-
BSG24_RS26580	58299..58943	-	645	WP_001310442.1	conjugal transfer protein TrbJ	-
BSG24_RS26585	58949..59944	-	996	WP_001028541.1	type IV secretion system protein	-
BSG24_RS26590	59948..60205	-	258	WP_000739144.1	hypothetical protein	-
BSG24_RS26595	60202..60504	-	303	WP_001360345.1	hypothetical protein	-
BSG24_RS26600	60520..60735	-	216	WP_021503156.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..65539	65539

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T68825 WP_000638823.1 NZ_CP018124:c56010-55729 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T68825 NZ_CP018124:c56010-55729 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT68825 WP_000121741.1 NZ_CP018124:c56251-56000 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT68825 NZ_CP018124:c56251-56000 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1