Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 54491..55092 Replicon plasmid pMRSN346638_67.9
Accession NZ_CP018117
Organism Escherichia coli strain MRSN346638

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag BSG23_RS26150 Protein ID WP_001216034.1
Coordinates 54491..54871 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag BSG23_RS26155 Protein ID WP_001190712.1
Coordinates 54871..55092 (-) Length 74 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSG23_RS26110 50734..51714 + 981 WP_001567628.1 IS5-like element IS5 family transposase -
BSG23_RS26115 51942..52259 + 318 WP_001513659.1 hypothetical protein -
BSG23_RS26120 52209..52517 - 309 Protein_61 DDE-type integrase/transposase/recombinase -
BSG23_RS26125 52534..53610 - 1077 WP_038989268.1 IS110-like element ISEc21 family transposase -
BSG23_RS28465 53853..53957 - 105 Protein_63 transposase -
BSG23_RS26140 53920..54279 - 360 WP_001513660.1 hypothetical protein -
BSG23_RS26145 54307..54486 - 180 WP_001513661.1 hypothetical protein -
BSG23_RS26150 54491..54871 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
BSG23_RS26155 54871..55092 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BSG23_RS26160 55275..56831 + 1557 WP_047633096.1 type I restriction-modification system subunit M -
BSG23_RS26165 56828..58075 + 1248 WP_001293038.1 restriction endonuclease subunit S -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul3 / ant(3'')-Ia / cmlA1 / aadA2 / blaCARB-2 / dfrA16 / tet(A) - 1..67946 67946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T68790 WP_001216034.1 NZ_CP018117:c54871-54491 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T68790 NZ_CP018117:c54871-54491 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT68790 WP_001190712.1 NZ_CP018117:c55092-54871 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT68790 NZ_CP018117:c55092-54871 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0AJ64


Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References