68784

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1966..2513 Replicon plasmid pMRSN346638_119.3
Accession NZ_CP018116
Organism Escherichia coli strain MRSN346638

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag BSG23_RS25015 Protein ID WP_000969996.1
Coordinates 2232..2513 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag BSG23_RS25010 Protein ID WP_000079928.1
Coordinates 1966..2235 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSG23_RS24995 26..1057 + 1032 WP_000907873.1 replication initiation protein -
BSG23_RS28710 1753..1911 + 159 WP_000841008.1 hypothetical protein -
BSG23_RS25010 1966..2235 + 270 WP_000079928.1 hypothetical protein Antitoxin
BSG23_RS25015 2232..2513 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BSG23_RS25020 2553..3401 + 849 WP_001057996.1 3'-5' exonuclease -
BSG23_RS28970 3638..3796 - 159 WP_197711125.1 MerR family transcriptional regulator -
BSG23_RS25025 3758..4939 + 1182 Protein_6 transposase -
BSG23_RS25030 5004..5708 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
BSG23_RS25035 5760..6236 + 477 Protein_8 recombinase family protein -
BSG23_RS25040 6419..7279 + 861 WP_032492330.1 class A broad-spectrum beta-lactamase TEM-57 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(A) / erm(42) / floR - 1..119254 119254


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T68784 WP_000969996.1 NZ_CP018116:2232-2513 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T68784 NZ_CP018116:2232-2513 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT68784 WP_000079928.1 NZ_CP018116:1966-2235 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT68784 NZ_CP018116:1966-2235 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References