Detailed information of TA system TA068783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4743891..4744467
• Replicon: chromosome
• Accession: NZ_CP018115
• Organism: Escherichia coli strain MRSN346638

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: BSG23_RS24675
• Protein ID: WP_001297643.1
• Coordinates: 4743891..4744178 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A080I7H7
• Locus tag: BSG23_RS24680
• Protein ID: WP_000063160.1
• Coordinates: 4744165..4744467 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSG23_RS24645	4740128..4741408	-	1281	WP_001037390.1	DUF445 domain-containing protein	-
BSG23_RS24650	4741449..4742681	-	1233	WP_001137030.1	multidrug efflux MFS transporter MdtM	-
BSG23_RS27720	4742996..4743692	+	697	Protein_4427	IS1 family transposase	-
BSG23_RS24675	4743891..4744178	+	288	WP_001297643.1	BrnT family toxin	Toxin
BSG23_RS24680	4744165..4744467	+	303	WP_000063160.1	BrnA antitoxin family protein	Antitoxin
BSG23_RS24685	4744501..4745457	-	957	WP_001297640.1	GTPase	-
BSG23_RS24690	4745468..4745671	-	204	WP_000467859.1	YbdD/YjiX family protein	-
BSG23_RS24695	4745721..4747871	-	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4743483..4743692	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T68783 WP_001297643.1 NZ_CP018115:4743891-4744178 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T68783 NZ_CP018115:4743891-4744178 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11552.01 Da
• Isoelectric Point: 9.4646

>AT68783 WP_000063160.1 NZ_CP018115:4744165-4744467 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT68783 NZ_CP018115:4744165-4744467 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A080I7H7