Detailed information of TA system TA068759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 54657..55179
• Replicon: plasmid pMRSN346595_64.5
• Accession: NZ_CP018112
• Organism: Escherichia coli strain MRSN346595

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: BSG21_RS26555
• Protein ID: WP_000638823.1
• Coordinates: 54657..54938 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: BSG21_RS26560
• Protein ID: WP_000121741.1
• Coordinates: 54928..55179 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSG21_RS26520	50806..51126	-	321	WP_000362081.1	VirB3 family type IV secretion system protein	-
BSG21_RS28855	51197..51487	-	291	WP_000865479.1	conjugal transfer protein	-
BSG21_RS26530	51487..52071	-	585	WP_063268637.1	lytic transglycosylase domain-containing protein	-
BSG21_RS26535	52092..52490	-	399	WP_071940825.1	hypothetical protein	-
BSG21_RS26540	52609..53046	-	438	WP_071940826.1	type IV pilus biogenesis protein PilM	-
BSG21_RS26545	53052..54287	-	1236	WP_071940827.1	TcpQ domain-containing protein	-
BSG21_RS26550	54290..54589	-	300	WP_000835764.1	IncN plasmid KikA protein	-
BSG21_RS26555	54657..54938	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BSG21_RS26560	54928..55179	-	252	WP_000121741.1	hypothetical protein	Antitoxin
BSG21_RS26565	55279..55914	-	636	WP_015059536.1	hypothetical protein	-
BSG21_RS26570	55962..56771	+	810	WP_001005148.1	hypothetical protein	-
BSG21_RS26575	56994..57218	-	225	WP_000713561.1	EexN family lipoprotein	-
BSG21_RS26580	57227..57871	-	645	WP_001310442.1	conjugal transfer protein TrbJ	-
BSG21_RS26585	57877..58872	-	996	WP_001028541.1	type IV secretion system protein	-
BSG21_RS26590	58876..59133	-	258	WP_000739144.1	hypothetical protein	-
BSG21_RS26595	59130..59432	-	303	WP_001360345.1	hypothetical protein	-
BSG21_RS26600	59448..59663	-	216	WP_021503156.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..64467	64467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T68759 WP_000638823.1 NZ_CP018112:c54938-54657 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T68759 NZ_CP018112:c54938-54657 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT68759 WP_000121741.1 NZ_CP018112:c55179-54928 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT68759 NZ_CP018112:c55179-54928 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1