Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 54491..55092 Replicon plasmid pMRSN346595_67.9
Accession NZ_CP018111
Organism Escherichia coli strain MRSN346595

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag BSG21_RS26135 Protein ID WP_001216034.1
Coordinates 54491..54871 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag BSG21_RS26140 Protein ID WP_001190712.1
Coordinates 54871..55092 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSG21_RS26095 50734..51714 + 981 WP_001567628.1 IS5-like element IS5 family transposase -
BSG21_RS26100 51942..52259 + 318 WP_001513659.1 hypothetical protein -
BSG21_RS26105 52209..52517 - 309 Protein_62 DDE-type integrase/transposase/recombinase -
BSG21_RS26110 52534..53610 - 1077 WP_038989268.1 IS110-like element ISEc21 family transposase -
BSG21_RS28550 53853..53957 - 105 Protein_64 transposase -
BSG21_RS26125 53920..54279 - 360 WP_001513660.1 hypothetical protein -
BSG21_RS26130 54307..54486 - 180 WP_001513661.1 hypothetical protein -
BSG21_RS26135 54491..54871 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
BSG21_RS26140 54871..55092 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BSG21_RS26145 55275..56831 + 1557 WP_047633096.1 type I restriction-modification system subunit M -
BSG21_RS26150 56828..58075 + 1248 WP_001293038.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul3 / ant(3'')-Ia / cmlA1 / aadA2 / blaCARB-2 / dfrA16 / tet(A) - 1..67946 67946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T68757 WP_001216034.1 NZ_CP018111:c54871-54491 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T68757 NZ_CP018111:c54871-54491 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT68757 WP_001190712.1 NZ_CP018111:c55092-54871 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT68757 NZ_CP018111:c55092-54871 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References