Detailed information of TA system TA068725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 6860..7361
• Replicon: plasmid pMR0716_mcr1
• Accession: NZ_CP018106
• Organism: Escherichia coli strain MRSN352231

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A1Z1UZ55
• Locus tag: BSG22_RS26945
• Protein ID: WP_052982837.1
• Coordinates: 6860..7120 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: BSG22_RS26950
• Protein ID: WP_000931278.1
• Coordinates: 7110..7361 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSG22_RS26895	2456..2617	+	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
BSG22_RS26900	2767..3363	+	597	WP_000125165.1	hypothetical protein	-
BSG22_RS30055	3381..3665	-	285	WP_001162371.1	hypothetical protein	-
BSG22_RS26910	3739..4404	-	666	WP_000532148.1	P-loop NTPase	-
BSG22_RS26920	5001..5330	+	330	WP_000855118.1	hypothetical protein	-
BSG22_RS26930	5911..6234	+	324	WP_000427402.1	hypothetical protein	-
BSG22_RS26935	6277..6486	+	210	WP_000866037.1	hypothetical protein	-
BSG22_RS26940	6584..6808	+	225	WP_000814384.1	hypothetical protein	-
BSG22_RS26945	6860..7120	+	261	WP_052982837.1	hypothetical protein	Toxin
BSG22_RS26950	7110..7361	+	252	WP_000931278.1	transcriptional regulator	Antitoxin
BSG22_RS28885	7432..7497	-	66	Protein_11	transposase	-
BSG22_RS26955	7522..8730	+	1209	WP_071940821.1	transposase	-
BSG22_RS26965	9247..9525	+	279	WP_000545938.1	hypothetical protein	-
BSG22_RS26970	9787..11013	-	1227	WP_071940830.1	transposase	-
BSG22_RS26975	11072..11476	+	405	WP_053001740.1	IS200/IS605 family transposase	-
BSG22_RS26985	11762..12028	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
BSG22_RS26990	12135..12317	-	183	WP_001481829.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..64467	64467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9907.48 Da
• Isoelectric Point: 10.0665

>T68725 WP_052982837.1 NZ_CP018106:6860-7120 [Escherichia coli]
MKIRKGDRQYYLNKEGDMFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVFMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T68725 NZ_CP018106:6860-7120 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATATGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTTTATGATGATTCAGGAAATGAGC
GAGGGAAAAAATGCTAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9420.81 Da
• Isoelectric Point: 9.8120

>AT68725 WP_000931278.1 NZ_CP018106:7110-7361 [Escherichia coli]
MLNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT68725 NZ_CP018106:7110-7361 [Escherichia coli]
ATGCTAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1Z1UZ55