Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 529293..529929 Replicon chromosome
Accession NZ_CP018100
Organism Bacillus safensis strain BRM1

Toxin (Protein)


Gene name mazF Uniprot ID A0A0P7G713
Locus tag BRL64_RS02610 Protein ID WP_024425388.1
Coordinates 529579..529929 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag BRL64_RS02605 Protein ID WP_003214273.1
Coordinates 529293..529574 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BRL64_RS02585 525433..526038 - 606 WP_041116754.1 rhomboid family intramembrane serine protease -
BRL64_RS02590 526133..526498 + 366 WP_041116756.1 holo-ACP synthase -
BRL64_RS02595 526659..527675 + 1017 WP_098677666.1 outer membrane lipoprotein carrier protein LolA -
BRL64_RS02600 527817..528998 + 1182 WP_081124016.1 alanine racemase -
BRL64_RS02605 529293..529574 + 282 WP_003214273.1 hypothetical protein Antitoxin
BRL64_RS02610 529579..529929 + 351 WP_024425388.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BRL64_RS02615 530045..530875 + 831 WP_034283948.1 RsbT co-antagonist protein RsbRA -
BRL64_RS02620 530880..531248 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
BRL64_RS02625 531251..531652 + 402 WP_024427376.1 anti-sigma regulatory factor -
BRL64_RS02630 531663..532670 + 1008 WP_024425391.1 PP2C family protein-serine/threonine phosphatase -
BRL64_RS02635 532730..533059 + 330 WP_024425392.1 anti-sigma factor antagonist -
BRL64_RS02640 533056..533544 + 489 WP_081124017.1 anti-sigma B factor RsbW -
BRL64_RS02645 533510..534298 + 789 WP_048240336.1 RNA polymerase sigma factor SigB -
BRL64_RS02650 534298..534897 + 600 WP_041089211.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8891

>T68688 WP_024425388.1 NZ_CP018100:529579-529929 [Bacillus safensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T68688 NZ_CP018100:529579-529929 [Bacillus safensis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTGATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTCTTGGAGCAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT68688 WP_003214273.1 NZ_CP018100:529293-529574 [Bacillus safensis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT68688 NZ_CP018100:529293-529574 [Bacillus safensis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTACCGGAGGCTTTGATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P7G713

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References