Detailed information of TA system TA068683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 694250..694827
• Replicon: chromosome
• Accession: NZ_CP018095
• Organism: Chelatococcus daeguensis strain TAD1

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BOQ54_RS03235
• Protein ID: WP_071923254.1
• Coordinates: 694250..694588 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BOQ54_RS03240
• Protein ID: WP_071923255.1
• Coordinates: 694576..694827 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOQ54_RS03200	689650..689880	+	231	WP_071923249.1	hypothetical protein	-
BOQ54_RS03205	690379..690930	+	552	WP_071923250.1	hypothetical protein	-
BOQ54_RS03210	690920..692620	+	1701	WP_071923251.1	relaxase/mobilization nuclease domain-containing protein	-
BOQ54_RS03215	692698..693315	+	618	WP_071923252.1	plasmid pRiA4b ORF-3 family protein	-
BOQ54_RS20135	693420..693539	+	120	Protein_635	type II toxin-antitoxin system ParD family antitoxin	-
BOQ54_RS20140	693609..693869	+	261	WP_071924382.1	nucleotidyltransferase family protein	-
BOQ54_RS03230	693869..694243	+	375	WP_071923253.1	DUF86 domain-containing protein	-
BOQ54_RS03235	694250..694588	-	339	WP_071923254.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BOQ54_RS03240	694576..694827	-	252	WP_071923255.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BOQ54_RS03245	694877..695269	-	393	WP_210187190.1	hypothetical protein	-
BOQ54_RS03250	695282..697027	-	1746	WP_210187191.1	type IV secretory system conjugative DNA transfer family protein	-
BOQ54_RS03255	697150..697377	-	228	WP_071923258.1	hypothetical protein	-
BOQ54_RS03260	697379..698032	-	654	WP_071923259.1	ParA family protein	-
BOQ54_RS03265	698149..698898	+	750	WP_071923260.1	LuxR family transcriptional regulator	-
BOQ54_RS03270	698995..699615	+	621	WP_210187192.1	conjugal transfer protein TraI	-
BOQ54_RS19630	699612..699797	+	186	WP_145979716.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	667261..720713	53452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12978.14 Da
• Isoelectric Point: 9.8830

>T68683 WP_071923254.1 NZ_CP018095:c694588-694250 [Chelatococcus daeguensis]
MGVVADVRRYDVYLVNLDPTLGSEIQKTRPCLVISPDEMNRHIRTVIIAPMTSVRRDYPTRVDVTFQRKKGQVVLDQIRT
VDKARLAKHLGRLPEPRCREVADLLQQMFAYE

Nucleotide

• Length: 339 bp

>T68683 NZ_CP018095:c694588-694250 [Chelatococcus daeguensis]
ATGGGAGTGGTAGCCGATGTCCGACGCTATGATGTGTATCTGGTGAACCTCGACCCGACGCTGGGCTCGGAGATCCAAAA
GACGCGACCGTGCCTGGTCATTTCGCCGGACGAGATGAACCGGCACATCCGCACGGTCATCATCGCCCCCATGACCTCCG
TGCGGCGTGACTACCCGACCCGCGTCGATGTCACCTTCCAACGGAAGAAGGGACAGGTCGTGCTCGACCAGATACGGACA
GTGGACAAGGCGCGCCTTGCCAAGCATCTCGGGCGCCTGCCGGAGCCACGTTGCCGCGAGGTCGCCGACCTGCTCCAGCA
GATGTTCGCCTACGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9377.67 Da
• Isoelectric Point: 4.8181

>AT68683 WP_071923255.1 NZ_CP018095:c694827-694576 [Chelatococcus daeguensis]
MKVNLIPIGNSKGVRIPSSVIKECGFGDQIEMRVEHGIVVLAPARRTRDGWDGAFARMAAAKDDTPLVPDTMEHDWDKEE
WEW

Nucleotide

• Length: 252 bp

>AT68683 NZ_CP018095:c694827-694576 [Chelatococcus daeguensis]
ATGAAAGTGAACCTGATCCCGATCGGCAATTCCAAGGGCGTGCGGATTCCCAGCTCGGTCATCAAGGAATGCGGCTTCGG
CGACCAGATCGAGATGCGCGTCGAGCACGGCATCGTCGTGCTGGCGCCCGCCCGTCGCACCAGAGATGGCTGGGACGGCG
CGTTCGCGAGGATGGCCGCAGCCAAGGACGACACCCCGCTTGTCCCCGACACGATGGAGCATGACTGGGATAAGGAGGAA
TGGGAGTGGTAG