Detailed information of TA system TA068676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 170349..170959
• Replicon: chromosome
• Accession: NZ_CP018091
• Organism: Synechococcus sp. SynAce01

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A1J0P7I5
• Locus tag: BM449_RS00935
• Protein ID: WP_071799710.1
• Coordinates: 170657..170959 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: BM449_RS00930
• Protein ID: WP_205526831.1
• Coordinates: 170349..170657 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BM449_RS00900	166412..166753	-	342	WP_071799704.1	type II toxin-antitoxin system PemK/MazF family toxin	-
BM449_RS00910	167085..168176	-	1092	Protein_187	IS5 family transposase	-
BM449_RS00915	168928..169188	-	261	WP_071799706.1	DUF2442 domain-containing protein	-
BM449_RS00920	169181..169414	-	234	WP_071799707.1	DUF4160 domain-containing protein	-
BM449_RS00925	169549..169902	-	354	WP_071799708.1	type II toxin-antitoxin system PemK/MazF family toxin	-
BM449_RS00930	170349..170657	-	309	WP_205526831.1	putative addiction module antidote protein	Antitoxin
BM449_RS00935	170657..170959	-	303	WP_071799710.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BM449_RS14825	171361..171771	+	411	WP_145537238.1	hypothetical protein	-
BM449_RS00940	172145..172762	-	618	WP_145537235.1	hypothetical protein	-
BM449_RS14830	172707..173354	-	648	WP_145537231.1	hypothetical protein	-
BM449_RS00945	173482..175071	-	1590	WP_145537228.1	RNA-directed DNA polymerase	-
BM449_RS14835	175177..175944	-	768	WP_145537223.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	159750..179268	19518
	-	flank	IS/Tn	-	-	167085..168176	1091
	-	inside	Integron	-	-	168844..171119	2275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11040.78 Da
• Isoelectric Point: 10.1314

>T68676 WP_071799710.1 NZ_CP018091:c170959-170657 [Synechococcus sp. SynAce01]
MVEVRQTERFVRWLVGLRDLRGRAKVLARIERLIGGNPGDVKPVGSGVSELRINYGPGYRVYYLKRGTTLIILLAGGDKS
SQAKDIDEALLLADNLTEAT

Nucleotide

• Length: 303 bp

>T68676 NZ_CP018091:c170959-170657 [Synechococcus sp. SynAce01]
ATGGTTGAAGTCCGCCAGACAGAGCGGTTCGTGCGTTGGCTTGTGGGTCTCCGTGATCTGAGAGGACGGGCGAAGGTTCT
GGCCAGGATCGAGCGCCTCATCGGCGGCAATCCTGGTGACGTCAAGCCCGTCGGGTCCGGCGTGTCGGAGTTGCGGATCA
ACTATGGCCCTGGATACAGGGTCTACTACCTGAAGAGGGGAACCACCTTGATCATCCTTTTGGCTGGCGGAGACAAGAGC
TCACAAGCCAAGGACATTGATGAAGCCCTTCTTTTGGCAGACAACCTGACGGAGGCGACCTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10825.20 Da
• Isoelectric Point: 4.3766

>AT68676 WP_205526831.1 NZ_CP018091:c170657-170349 [Synechococcus sp. SynAce01]
MKTTTSLYDVAEHLQTPEDMAAYLEACIEEADGDAAFIAKALGDIARAQGMTQVARDSGLSRESLYKALSGERSPSFDTI
LKVVTALGLKLSAGVRSEAEVT

Nucleotide

• Length: 309 bp

>AT68676 NZ_CP018091:c170657-170349 [Synechococcus sp. SynAce01]
ATGAAAACTACGACCAGCCTGTACGACGTTGCCGAGCATCTCCAAACACCGGAGGATATGGCGGCCTATCTGGAAGCCTG
CATCGAGGAGGCTGATGGTGATGCTGCCTTCATTGCAAAGGCTCTGGGCGACATCGCACGTGCCCAGGGGATGACCCAGG
TGGCACGAGATTCGGGTCTTTCCAGGGAGAGTCTCTACAAGGCCCTGTCTGGTGAGCGCAGCCCGAGCTTTGACACCATC
CTGAAAGTCGTCACGGCTCTTGGACTGAAGTTGAGCGCCGGCGTGAGAAGTGAAGCGGAGGTGACTTGA

Structures

Toxin

• AlphaFold DB: A0A1J0P7I5