Detailed information of TA system TA068675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 115756..116390
• Replicon: chromosome
• Accession: NZ_CP018091
• Organism: Synechococcus sp. SynAce01

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BM449_RS00620
• Protein ID: WP_186437673.1
• Coordinates: 115756..116058 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A1J0P730
• Locus tag: BM449_RS00625
• Protein ID: WP_071799670.1
• Coordinates: 116058..116390 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BM449_RS00605	111120..111737	+	618	WP_071799666.1	DUF1788 domain-containing protein	-
BM449_RS00610	111734..115219	+	3486	WP_071799667.1	BREX system P-loop protein BrxC	-
BM449_RS15130	115570..115737	+	168	WP_186437671.1	hypothetical protein	-
BM449_RS00620	115756..116058	+	303	WP_186437673.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BM449_RS00625	116058..116390	+	333	WP_071799670.1	putative addiction module antidote protein	Antitoxin
BM449_RS00630	116441..118246	+	1806	WP_162922806.1	BREX-1 system adenine-specific DNA-methyltransferase PglX	-
BM449_RS00635	118210..119148	-	939	WP_071799672.1	IS481 family transposase	-
BM449_RS00640	119327..119515	-	189	WP_071799673.1	hypothetical protein	-
BM449_RS00645	119988..120293	+	306	WP_071801801.1	MTH1187 family thiamine-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (58-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11283.87 Da
• Isoelectric Point: 9.3924

>T68675 WP_186437673.1 NZ_CP018091:115756-116058 [Synechococcus sp. SynAce01]
MMRVVKSEVYQAWIDGLRDQAGRARVLMRVDRLIHGNPGDYRNLTEGVSELKIDVGPGYRVYYARRGDHLLLLLGGGSKA
TQAKDIAKALDLNREFEDHS

Nucleotide

• Length: 303 bp

>T68675 NZ_CP018091:115756-116058 [Synechococcus sp. SynAce01]
CTGATGAGGGTCGTCAAGTCGGAGGTCTACCAAGCGTGGATCGACGGCCTCAGGGATCAAGCCGGCAGGGCTCGAGTGTT
GATGCGAGTGGATCGCTTGATCCATGGCAACCCCGGCGACTATCGCAATCTCACGGAGGGAGTGTCTGAACTGAAAATCG
ATGTCGGACCCGGCTACCGGGTGTATTACGCCCGAAGAGGCGATCACCTGCTTCTGCTGTTGGGCGGGGGCAGTAAAGCG
ACCCAGGCCAAAGACATCGCCAAGGCTCTTGATTTAAACCGTGAATTTGAGGATCACTCGTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 11721.24 Da
• Isoelectric Point: 5.6007

>AT68675 WP_071799670.1 NZ_CP018091:116058-116390 [Synechococcus sp. SynAce01]
MNQAQPLANPSATRFTPYDVAEHLRTPDEMAAYLDAWLEEAPDDVAGLARALGDIARAKGMTQVAKDTGLSRESLYRALS
ADGNPSFATVLKVTKALGLRLHAHAVACEG

Nucleotide

• Length: 333 bp

>AT68675 NZ_CP018091:116058-116390 [Synechococcus sp. SynAce01]
ATGAACCAAGCCCAACCCCTTGCTAATCCATCGGCAACCAGGTTCACGCCCTATGACGTGGCCGAGCATCTCAGAACGCC
AGATGAGATGGCGGCCTATCTGGATGCCTGGCTTGAGGAAGCACCGGATGATGTGGCCGGTCTTGCACGGGCCTTGGGGG
ACATTGCCCGAGCCAAGGGGATGACACAGGTGGCGAAAGACACGGGCCTCAGCCGGGAGAGCCTTTATCGGGCGCTCAGC
GCCGATGGCAATCCAAGCTTCGCCACCGTGCTGAAGGTGACCAAGGCTCTGGGCTTGCGGCTTCATGCCCATGCCGTTGC
TTGTGAGGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1J0P730