Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 18889..20026 Replicon plasmid unnamed2
Accession NZ_CP018072
Organism Enterococcus faecium strain VRE001

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag BOW68_RS16110 Protein ID WP_001284311.1
Coordinates 19163..20026 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag BOW68_RS16105 Protein ID WP_000301765.1
Coordinates 18889..19161 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOW68_RS16070 14457..14819 - 363 Protein_16 replication initiation protein -
BOW68_RS17900 15494..15589 + 96 WP_106913803.1 type I toxin-antitoxin system Fst family toxin -
BOW68_RS16075 16289..16573 + 285 WP_000353844.1 hypothetical protein -
BOW68_RS16080 16576..16992 + 417 WP_001232859.1 hypothetical protein -
BOW68_RS16085 17129..17497 - 369 Protein_20 replication initiation protein -
BOW68_RS16090 17572..18252 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
BOW68_RS16095 18308..18565 + 258 WP_002347171.1 hypothetical protein -
BOW68_RS16100 18663..18872 + 210 WP_000527318.1 peptide-binding protein -
BOW68_RS16105 18889..19161 + 273 WP_000301765.1 antitoxin Antitoxin
BOW68_RS16110 19163..20026 + 864 WP_001284311.1 toxin zeta Toxin
BOW68_RS16115 20289..21026 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
BOW68_RS16120 21151..21234 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
BOW68_RS17735 21283..21366 - 84 Protein_28 peptide-binding protein -
BOW68_RS16125 21776..22570 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
BOW68_RS16130 22663..22914 - 252 Protein_30 GNAT family N-acetyltransferase -
BOW68_RS16135 23159..24148 + 990 Protein_31 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(L) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 1..59226 59226
- inside IScluster/Tn tet(L) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) - 4542..58251 53709


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T68663 WP_001284311.1 NZ_CP018072:19163-20026 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T68663 NZ_CP018072:19163-20026 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT68663 WP_000301765.1 NZ_CP018072:18889-19161 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT68663 NZ_CP018072:18889-19161 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References