Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 2213936..2214465 Replicon chromosome
Accession NZ_CP018071
Organism Enterococcus faecium strain VRE001

Toxin (Protein)


Gene name relE Uniprot ID Q3XX67
Locus tag BOW68_RS12175 Protein ID WP_002296560.1
Coordinates 2213936..2214202 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A829F3T0
Locus tag BOW68_RS12180 Protein ID WP_002296559.1
Coordinates 2214202..2214465 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOW68_RS12145 2209304..2210479 + 1176 WP_002294192.1 betaine/proline/choline family ABC transporter ATP-binding protein -
BOW68_RS12150 2210483..2211124 + 642 WP_002293405.1 ABC transporter permease -
BOW68_RS12155 2211121..2212038 + 918 WP_002296564.1 osmoprotectant ABC transporter substrate-binding protein -
BOW68_RS12160 2212035..2212706 + 672 WP_002302663.1 ABC transporter permease -
BOW68_RS12170 2213247..2213735 - 489 WP_002296561.1 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase -
BOW68_RS12175 2213936..2214202 - 267 WP_002296560.1 Txe/YoeB family addiction module toxin Toxin
BOW68_RS12180 2214202..2214465 - 264 WP_002296559.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BOW68_RS12190 2214941..2215126 + 186 WP_002296558.1 transcriptional regulator -
BOW68_RS12195 2215268..2217091 - 1824 WP_002294202.1 APC family permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10660.13 Da        Isoelectric Point: 9.9931

>T68662 WP_002296560.1 NZ_CP018071:c2214202-2213936 [Enterococcus faecium]
MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T68662 NZ_CP018071:c2214202-2213936 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10046.05 Da        Isoelectric Point: 4.1207

>AT68662 WP_002296559.1 NZ_CP018071:c2214465-2214202 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNID
DIDWEAL

Download         Length: 264 bp

>AT68662 NZ_CP018071:c2214465-2214202 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FCW8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3T0

References