Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1923993..1924564 Replicon chromosome
Accession NZ_CP018071
Organism Enterococcus faecium strain VRE001

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag BOW68_RS10680 Protein ID WP_002286801.1
Coordinates 1923993..1924334 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag BOW68_RS10685 Protein ID WP_002323011.1
Coordinates 1924334..1924564 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOW68_RS10645 1919203..1920537 - 1335 WP_002303373.1 ABC transporter substrate-binding protein -
BOW68_RS10650 1920745..1921575 + 831 WP_002286789.1 manganese catalase family protein -
BOW68_RS17600 1921819..1922101 - 283 Protein_1840 transposase -
BOW68_RS10665 1922259..1922453 - 195 WP_002297028.1 hypothetical protein -
BOW68_RS10675 1922958..1923143 - 186 WP_002304207.1 hypothetical protein -
BOW68_RS10680 1923993..1924334 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BOW68_RS10685 1924334..1924564 - 231 WP_002323011.1 hypothetical protein Antitoxin
BOW68_RS10695 1924851..1925189 + 339 WP_002286804.1 hypothetical protein -
BOW68_RS10700 1925394..1925633 - 240 WP_002321938.1 SOS response-associated peptidase family protein -
BOW68_RS10705 1925609..1925956 - 348 WP_002286808.1 SOS response-associated peptidase family protein -
BOW68_RS10710 1926688..1927269 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
BOW68_RS10715 1927452..1928078 - 627 WP_002286816.1 cysteine hydrolase -
BOW68_RS10720 1928100..1929431 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T68661 WP_002286801.1 NZ_CP018071:c1924334-1923993 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T68661 NZ_CP018071:c1924334-1923993 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT68661 WP_002323011.1 NZ_CP018071:c1924564-1924334 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT68661 NZ_CP018071:c1924564-1924334 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References