Detailed information of TA system TA068660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 33453..33972
• Replicon: plasmid unnamed1
• Accession: NZ_CP018070
• Organism: Enterococcus faecium strain VRE001

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: BOW68_RS00220
• Protein ID: WP_000588503.1
• Coordinates: 33453..33710 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: BOW68_RS00225
• Protein ID: WP_000388479.1
• Coordinates: 33703..33972 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOW68_RS00195	29544..30128	-	585	WP_002299575.1	recombinase family protein	-
BOW68_RS00200	30339..30542	-	204	WP_002299573.1	hypothetical protein	-
BOW68_RS00205	30552..30974	-	423	WP_002303116.1	hypothetical protein	-
BOW68_RS00210	31212..31826	+	615	WP_001261742.1	recombinase family protein	-
BOW68_RS00215	31991..32815	-	825	WP_001809248.1	hypothetical protein	-
BOW68_RS17955	32839..32994	-	156	WP_002347142.1	hypothetical protein	-
BOW68_RS00220	33453..33710	-	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
BOW68_RS00225	33703..33972	-	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BOW68_RS00230	34103..34282	-	180	WP_001120991.1	hypothetical protein	-
BOW68_RS00240	34663..35370	-	708	WP_002303117.1	hypothetical protein	-
BOW68_RS00245	35363..35800	-	438	WP_002300851.1	hypothetical protein	-
BOW68_RS00250	35815..36066	-	252	WP_002296238.1	hypothetical protein	-
BOW68_RS00255	36066..36272	-	207	WP_002347218.1	hypothetical protein	-
BOW68_RS00265	36709..36969	-	261	WP_002295623.1	hypothetical protein	-
BOW68_RS00270	37044..37247	+	204	WP_002316074.1	putative holin-like toxin	-
BOW68_RS00275	37388..37873	-	486	WP_002303313.1	single-stranded DNA-binding protein	-
BOW68_RS00280	37876..38775	-	900	WP_002303312.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..78626	78626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T68660 WP_000588503.1 NZ_CP018070:c33710-33453 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T68660 NZ_CP018070:c33710-33453 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT68660 WP_000388479.1 NZ_CP018070:c33972-33703 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT68660 NZ_CP018070:c33972-33703 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7